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ProteomicsLarge-scale qualitative and quantitative research of dityrosine crosslinking omics in Escherichia coli
Excessive reactive oxygen species cause oxidative stress in cells. Two molecules of tyrosine can generate dityrosine, which is not only a product of natural posttranslational processes, but also a putative biomarker for protein oxidation. Few studies have investigated dityrosine crosslinking under e...
ORGANISM(S): Escherichia coli 
2023-05-10 | PXD039132 | Pride
Hydrogen peroxide Dityrosine crosslinking Label-free proteomics 
ProteomicsQuantitative photo-crosslinking mass spectrometry reveals protein structure response to environmental changes
Conformational changes of protein structures depend on their chemical and physical environment. Studying conformational changes depending on environmental parameters is notoriously difficult as many methods of structural biology are affected by parameters like temperature or pH. To make such conform...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2019-07-01 | PXD012939 | Pride
Data-independent acquisition Structural dynamics Human serum albumin Dia-qclms Quantitation Xi Cytochrome c Spectronaut Label-free Mass spectrometry Photo-crosslinking Cross-linking Sda 
MetabolomicsMetabolomics profiling (GC-MS and LC-MS) for time-course differentiation of pre-B cells-like (B3 cell line) under the controlled induction of the transcription factor Ikaros (STATegra Project)
The main goal of the project is to develop a new generation of bioinformatics resources for the integrative analysis of multiple types of 'omics data. These resources include both novel statistical methodologies as well as user-friendly software implementations. STATegra methods address many aspects...
2016-12-15 | MTBLS283 | MetaboLights
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo sapiens (Human) 
2016-04-27 | PXD003754 | Pride
Proteomics  protein complexes  protein crosslinking  mass spectrometry Membrane protein  interactome analysis 
ProteomicsClick-linking: a cell-compatible protein crosslinking method based on click chemistry
We developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive l...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-01-28 | PXD059118 | Pride
In situ Crosslinking Proteome-wide Protein-protein interactions Interactome 
ProteomicsIdentifying subcellular structure components in Escherichia Coli by crosslinking and SEC-MS
Cells are comprised of a broad spectrum of structures that compartmentalize biochemical and signaling mechanisms. These structures can be comprised of many biomolecules, but especially lipids, proteins, and nucleic acids. Options are limited to quantify or discover new subcellular structures. We exp...
ORGANISM(S): Escherichia coli 
2026-05-25 | PXD054099 | Pride
Size-exclusion chromatography and mass spectrometry 
Proteomicsquantitative crosslinking of ADP treated mitochondria isolated from HEK293 cells
Acute treatment of isolated mitochondria with 1.5 mM ADP. Crosslinking and quantitative comparison to non-treated control using iqPIR technology for quantitative crosslinking.
ORGANISM(S): Homo sapiens (Human) 
2022-07-29 | PXD031644 | Pride
Hek293 Qxl-ms Mitochondria 
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo sapiens (Human) 
2022-03-01 | PXD002929 | Pride
Crosslinking Protein-protein interactions 
ProteomicsReliable identification of protein-protein interactions by crosslinking mass spectrometry_AP-MS
Quantitative proteomics dataset on soluble, affinity-enriched proteins from SPA-pulldowns in E. coli K12, on rpoB-SPA / nusG-SPA / yacL-SPA. This data was used to identify proteins for enrichment analysis and subsequent crosslinking mass spectrometry search (DB creation). Cleared E. coli lysate was ...
ORGANISM(S): Escherichia Coli 
2021-04-14 | PXD024146 | JPOST Repository
UnknownUnraveling the surface glycoprotein interaction network by integrating chemical crosslinking with MS-based proteomics.
Not available
S-EPMC8179341 | biostudies-literature
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