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TranscriptomicsAAV vector-mediated in vivo miRNA antagonism for studying miRNA function and treating dyslipidemia
Understanding the function of individual miRNA species in mice would require the production of hundreds of loss-of-function strains. To accelerate analysis of miRNA biology in mammals, we combined recombinant adeno-associated virus (rAAV) vectors with miRNA ‘Tough Decoys’ (TuDs) to inhibit specific ...
ORGANISM(S): Mus musculus 
2011-12-10 | E-GEOD-25971 | biostudies-arrayexpress
TranscriptomicsTranscriptional changes induced by rAAV-mediated Npas4 overexpression in hippocampal primary cell culture compared to GFP-control
Hippocampal neuron cultures were prepared from neonatal C57BL/6N mice as previously described (Bading and Greenberg, Science, 1991). Cells were plated in dishes coated with Poly-D-Lysine (2mg/ml) and Laminin (10 µg/ml). Two days prior to harvesting, the medium was changed from NBA to transfection me...
ORGANISM(S): Mus musculus 
2021-10-18 | E-MTAB-10706 | biostudies-arrayexpress
ProteomicsAAV production system proteomics
We performed absolute quantification (AQUA) of viral proteins by targeted quantitative proteomics and conducted tandem mass tag (TMT)-based proteomics on the three rAAV and wtAAV production systems to identify potential factors limiting rAAV productivity in HEK293 cells
ORGANISM(S): Homo sapiens (Human) 
2024-07-03 | PXD050484 | Pride
Raav Proteomics Wtaav 
ProteomicsAnalysis of the nuclear proteome fraction of rAAV2-plasmid transfected HEK-293 cells during AAV production
In this comparative proteomic analysis, we focused on proteins in the nuclear fraction. As the nucleus is the epicenter of DNA uptake, transcription, capsid assembly and packaging. Three HEK-293 cell samples i) not transfected, ii) MOCK plasmid transfected, and iii) triple-transfected with rAAV2-pro...
ORGANISM(S): Homo sapiens (Human) 
2025-07-21 | PXD062079 | Pride
Ms/ms Nuclear fraction Fasp Raav production 
UnknownCRISPR screen reveals modifiers of rAAV production including known rAAV infection genes playing an unexpected role in vector production.
Not available
S-EPMC11840188 | biostudies-literature
GenomicsProduction of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293
Production of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293
PRJNA930839 | ENA
UnknownReduced rAAV interference enhances rcAAV detection sensitivity.
Not available
S-EPMC12475838 | biostudies-literature
Genomics
Intrathymic delivery of rAAV
PRJNA832955 | ENA
UnknownCrucial aspects for maintaining rAAV stability.
Not available
S-EPMC11557909 | biostudies-literature
TranscriptomicsProduction of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293
To understand the response of host cells to rAAV vector production via plasmid transfection, samples were collected throughout the course of three 50 L production batches and analyzed by bulk RNA sequencing of polyadenylated transcripts (RNA-seq). Gene ontology analysis determined that upregulated p...
ORGANISM(S): Homo sapiens 
2023-02-06 | GSE224405 | GEO
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