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ProteomicsAnalysis of the nuclear proteome fraction of rAAV2-plasmid transfected HEK-293 cells during AAV production
In this comparative proteomic analysis, we focused on proteins in the nuclear fraction. As the nucleus is the epicenter of DNA uptake, transcription, capsid assembly and packaging. Three HEK-293 cell samples i) not transfected, ii) MOCK plasmid transfected, and iii) triple-transfected with rAAV2-pro...
ORGANISM(S): Homo sapiens (Human) 
2025-07-21 | PXD062079 | Pride
Ms/ms Nuclear fraction Fasp Raav production 
ProteomicsAAV production system proteomics
We performed absolute quantification (AQUA) of viral proteins by targeted quantitative proteomics and conducted tandem mass tag (TMT)-based proteomics on the three rAAV and wtAAV production systems to identify potential factors limiting rAAV productivity in HEK293 cells
ORGANISM(S): Homo sapiens (Human) 
2024-07-03 | PXD050484 | Pride
Raav Proteomics Wtaav 
GenomicsProduction of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293
Production of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293
PRJNA930839 | ENA
TranscriptomicsAAV vector-mediated in vivo miRNA antagonism for studying miRNA function and treating dyslipidemia
Understanding the function of individual miRNA species in mice would require the production of hundreds of loss-of-function strains. To accelerate analysis of miRNA biology in mammals, we combined recombinant adeno-associated virus (rAAV) vectors with miRNA ‘Tough Decoys’ (TuDs) to inhibit specific ...
ORGANISM(S): Mus musculus 
2011-12-10 | E-GEOD-25971 | biostudies-arrayexpress
TranscriptomicsProduction of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293
To understand the response of host cells to rAAV vector production via plasmid transfection, samples were collected throughout the course of three 50 L production batches and analyzed by bulk RNA sequencing of polyadenylated transcripts (RNA-seq). Gene ontology analysis determined that upregulated p...
ORGANISM(S): Homo sapiens 
2023-02-06 | GSE224405 | GEO
UnknownCRISPR screen reveals modifiers of rAAV production including known rAAV infection genes playing an unexpected role in vector production.
Not available
S-EPMC11840188 | biostudies-literature
UnknownPerformance of Cardiotropic rAAV Vectors Is Dependent on Production Method.
Not available
S-EPMC9341392 | biostudies-literature
UnknownCRISPR screen for rAAV production implicates genes associated with infection.
Not available
S-EPMC11429925 | biostudies-literature
GenomicsComprehensive mRNA-sequencing-based characterization of three HEK-293 cell lines during an rAAV production process for Gene Therapy Applications
Comprehensive mRNA-sequencing-based characterization of three HEK-293 cell lines during an rAAV production process for Gene Therapy Applications
PRJNA938438 | ENA
UnknownrAAV Production and Titration at the Microscale for High-Throughput Screening.
Not available
S-EPMC8819507 | biostudies-literature
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