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ProteomicsCell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain
Proteomic profiling of brain cell types using isolation-based strategies pose limitations in resolving cellular phenotypes representative of their native state. We generated a novel mouse line for cell type-specific expression of biotin ligase TurboID, leading to in vivo biotinylation of proteins. U...
ORGANISM(S): Mus musculus (Mouse) 
2022-04-28 | PXD032161 | Pride
Mouse Brain Proteomics Lc-ms/ms 
ProteomicsIdentification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles
We performed quantitative label-free quantitative mass spectrometry (LFQ-MS) on 16 cell lysates and 16 EV lysates derived from BV2 microglia. These included four groups of BV2 cells (n=4/group) that were treated (72 hours) with either LPS (100ng/mL) to polarize to a pro-inflammatory state, IL-10 (50...
ORGANISM(S): Mus musculus (Mouse) 
2023-11-21 | PXD043959 | Pride
Neuroinflammation Proteomics Mass spectrometry Extracellular vesicles Microglia Transcriptomics Rna sequencing 
ProteomicsMolecular signatures of neuroinflammation induced by α-synuclein aggregates-A multi-proteomics approach (Mouse Arm)
Alpha-synuclein aggregates (αSynAgg) are pathological hallmarks of Parkinson’s disease (PD) that induce microglial activation and immune-mediated neurotoxicity, although the molecular mechanisms of αSynAgg-induced immune activation are poorly defined. We used mass spectrometry to define proteomic ch...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-24 | PXD013691 | Pride
Snca Mouse Microglia Alpha synuclein aggregates Parkinsons disease 
ProteomicsSimultaneous profiling of native-state proteomes and transcriptomes of brain cell types using proximity labeling
Deep molecular phenotyping of cells at transcriptomic and proteomic levels is an essential first step to understanding cellular contributions to development, aging, injury, and disease. Since proteome and transcriptome level abundances only modestly correlate with each other, complementary profiling...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-02-16 | PXD073954 | Pride
Proximity labeling  transcriptomics  proteomics  microglia  astrocytes  neurons  mrna-protein concordance 
ProteomicsCellular proteomic profiling using proximity labelling by TurboID-NES in microglial and neuronal cell lines.
Different brain cell types play distinct roles in brain development and disease via cellular mechanisms. Molecular characterization of these cellular mechanisms using cell type-specific approaches, particularly at the protein (proteomic) level, can provide biological and therapeutic insights. Conven...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-11 | PXD036744 | Pride
Bv2 N2a Inflammation Proteomics Turboid 
ProteomicsNative-state proteomics of Parvalbumin interneurons identifies novel molecular signatures and metabolic vulnerabilities to early Alzheimer's disease pathology
One of the earliest pathophysiological perturbations in Alzheimer’s Disease (AD) may arise from dysfunction of fast-spiking parvalbumin (PV) interneurons (PV-INs). Defining early protein-level (proteomic) alterations in PV-INs can provide key biological and translationally relevant insights. Here, w...
ORGANISM(S): Mus musculus (Mouse) 
2024-03-04 | PXD043963 | Pride
Alzheimer’s disease Parvalbumin interneurons Proteomics 
ProteomicsCell type-specific in vivo proximity labeling of the neuronal proteome reveals regional differences in mouse brain.
Isolation and proteomic profiling of brain cell types, particularly neurons, pose several technical challenges which limit our ability to resolve distinct cellular phenotypes in neurological diseases. Therefore, we generated a novel mouse line that enables cell type-specific expression of a biotin l...
ORGANISM(S): Mus musculus (Mouse) 
2022-04-28 | PXD027488 | Pride
Mass spectrometry Mouse Proteome Lc-ms/ms Turboid 
ProteomicsSimultaneous profiling of native-state proteomes and transcriptomes of brain cell types using proximity labeling
Deep molecular phenotyping of cells at transcriptomic and proteomic levels is an essential first step to understanding cellular contributions to development, aging, injury, and disease. Since proteome and transcriptome level abundances only modestly correlate with each other, complementary profiling...
ORGANISM(S): Mus musculus (Mouse) 
2026-02-16 | PXD059818 | Pride
Proximity labeling  transcriptomics  proteomics  microglia  astrocytes  neurons  mrna-protein concordance 
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