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ProteomicsChromatin-associated protein complexes link DNA base J and transcription termination in Leishmania
Tandem affinity purification mass spectrometry of protein complexes regulating DNA Base J synthesis in Leishmania
ORGANISM(S): Leishmania Tarentolae (ncbitaxon:5689) 
2020-08-06 | MSV000085924 | MassIVE
ProteomicsArchitecture of the chromatin remodeler RSC and insights into its nucleosome engagement
Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in geno...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-05-27 | PXD019400 | Pride
Yrsc cxms cryo-em 
ProteomicsYeast SWI/SNF complex - Loss of Snf5 Induces Formation of an Aberrant SWI/SNF Complex
This project uses crosslinked based mass spectrometry (CXMS) to study the architecture of yeast SWI/SNF complex.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-06-14 | PXD005948 | Pride
Swi/snf bs3 crosslinking architecture 
ProteomicsHistone octamer structure is altered early in ISW2 ATP-dependent remodeling of nucleosomes
Histone octamers are thought to be a rigid part of nucleosomes that shape chromatin and block cellular machinery from accessing DNA. ATP-dependent chromatin remodelers like ISW2 mobilize nucleosomes to provide DNA access. We find evidence for histone octamer distortion preceding DNA being moved into...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2020-05-27 | PXD013967 | Pride
Transcription Nucleosome structure Nucleosome dynamics Histones Chromatin remodeling Sant domain Gene regulation Iswi Nucleosome movement 
ProteomicsUnique structural features of the human NuA4/TIP60 acetyltransferase and chromatin remodeling complex
The human NuA4/TIP60 co-activator complex, a fusion of the yeast SWR1 and NuA4 complexes, both incorporates the histone variant H2A.Z into nucleosomes and acetylates histones H4/H2A/H2A.Z to play crucial roles regulating gene expression and maintaining genome stability. Our cryo-EM studies show that...
ORGANISM(S): Homo sapiens (Human) 
2024-08-08 | PXD053209 | Pride
Nua4/tip60 acetyltransferase P400-ncp complex H2a.z Nua4 and swr1 chromatin modifier and remodeler complexes 
ProteomicsTAF8 regions important for TFIID lobe B assembly are required for embryonic stem cell survival
The human general transcription factor TFIID is composed of the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). In eukaryotic cells, TFIID is thought to nucleate RNA polymerase II (Pol II) preinitiation complex formation on all protein coding gene promoters and thus, be crucial for ...
ORGANISM(S): Homo sapiens (Human) 
2021-10-02 | PXD026575 | Pride
Embryonic stem cell (esc) Taf8 Tfiid Cxms 
ProteomicsHuman BAF complex (HA-DPF2 pulldown and SS18-Flag pulldown)
Mammalian SWI/SNF (mSWI/SNF) ATP-dependent chromatin remodeling complexes are large, multisubunit molecular machines that play vital roles in regulating genomic architecture and are frequently disrupted in human cancer and developmental disorders.Using affinity purification of endogenous complexes f...
ORGANISM(S): Homo sapiens (Human) 
2018-10-18 | PXD010124 | Pride
Bs3 crosslinking Baf complex Hswi/snf 
ProteomicsSWI/SNF is positively auto-regulated by an AT-hook in the catalytic subunit
The SWI/SNF ATP-dependent chromatin remodeler is a master regulator of the epigenome; controlling pluripotency, cell fate determination and differentiation. There is a sparsity of information on the autoregulation of SWI/SNF, the domains involved and their mode of action. We find a DNA or RNA bindin...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-06-15 | PXD040664 | Pride
Swi/snf at-hook nucleosomes 
ProteomicsA structural model of the human mSWI/SNF (BAF) complex bound to the nucleosome informs disease mechanisms
Mammalian SWI/SNF complexes are multi-component ATP-dependent chromatin-remodeling complexes that regulate genomic architecture. Here we present the first structural model of the human canonical BAF chromatin-remodeling complex bound to a nucleosome generated using cryo-EM, cross-linking mass spectr...
ORGANISM(S): Homo sapiens (Human) 
2021-02-10 | PXD020992 | Pride
Homology model Cryo-em Atp-dependent chromatin remodeling Nucleosome acidic patch Yeast swi/snf Mammalian swi/snf Canonical baf complex Human disease Mutations 
ProteomicsYang_NuA4_TIP60_structural_features
This submission contains the mass spectrometry files for the manuscript by Zhenlin Yang et al. that describes the unique structural features of the NuA4/TIP60 acetyltransferase and chromatin remodeling complex. MS experiments were performed from TAP purified complexes from K562 cells and MS files we...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-02 | MSV000093280 | MassIVE
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