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MetabolomicsIsotope Labeled Reactivity-Based Metabolomics Reveal Cysteine as a Potent In Vivo Scavenger of Lactoylglutathione

Metabolite adducts are increasingly being recognized as important intermediates in cellular metabolism and as circulating messengers. Methylglyoxal (MG) is a highly reactive metabolite involved in diabetes and aging, via formation of a wide variety of protein adducts. Though new types of protein ...

2025-04-29 | MTBLS6287 | MetaboLights
ProteomicsChemoselective umpolung of thiols to episulfoniums for cysteine bioconjugation
Modification of native cysteine is an important tool in protein research and relies on fast and chemoselective reactions under bioambient conditions. Nucleophilic cysteine can be functionalized with electrophiles, such as maleimides, that carry specific chemical groups, depending on the desired appl...
ORGANISM(S): Escherichia Coli 
2023-12-20 | PXD038723 | JPOST Repository
ProteomicsPervasive aggregation and depletion of host and viral proteins in response to cysteine-reactive electrophilic compounds
isoTOP-ABPP performed for a small library of cysteine-reactive compounds to assess cysteine ligandability of viral and host proteins towards these compounds.
ORGANISM(S): Homo sapiens (Human) 
2024-10-24 | PXD046278 | Pride
Isotop-abpp  sars-cov-2  cysteine-reactive compounds  proteostasis  orbitrap eclipse: sp3  faims 
TranscriptomicsDepleting cytosolic cysteine compromises the antioxidant capacity of the cytosol in Arabidopsis thaliana
Plant cells contain different O-acetylserine(thiol)lyase (OASTL) enzymes involved in Cys biosynthesis and located in different subcellular compartments. These enzymes are made up of a complex variety of isoforms resulting in different subcellular Cys pools. To unravel the contribution of cytosolic C...
ORGANISM(S): Arabidopsis thaliana 
2010-10-01 | E-GEOD-19245 | biostudies-arrayexpress
ProteomicsExploring Alternative Cysteine-reactive Chemistry for MS-cleavable Cross-linking Mass Spectrometry
To enable global PPI mapping, we have explored an alternative cysteine labeling chemistry, and thus designed and synthesized a sulfoxide-containing MS-cleavable haloacetamide-based cross-linker, DiBromoAcetamide SulfOxide (DBrASO). In combination with a newly developed SEC-HpHt fractionation method,...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD037443 | Pride
Cysteine Proteomics Cross-linking mass spectrometry 
ProteomicsCRK2-mediated control of ROS production by phosphorylation of the RBOHD C-terminus in Arabidopsis
Reactive oxygen species (ROS) are important messengers in eukaryotic organisms. Extracellular ROS production by NADPH oxidases in plants is triggered by receptor-like protein kinase (RLK)-dependent signaling networks. This ROS production is tightly controlled by multiple mechanisms including phospho...
ORGANISM(S): Arabidopsis Thaliana 
2020-03-27 | PXD013525 | panorama
TranscriptomicscDNA microarray assay of lymph node biopsies diagnosed as lymphoma or as reactive tissue
Gene expression profiling of biopsied human lymph node (LN) tissue comparing each patient sample against mobilised peripheral blood stem cells (PBSC), the reference channel Evaluate whether gene expression microarray can diagnose lymph node biopsies as reactive or as one of three main types of lymph...
ORGANISM(S): Homo sapiens 
2012-11-28 | E-GEOD-23647 | biostudies-arrayexpress
ProteomicsAccelerating multiplexed profiling of protein-ligand interactions: high-throughput plate-based reactive cysteine profiling with minimal input
Chemoproteomics investigates small molecule-protein interactions and has made significant progress in recent years. Despite its vast potential, the proteome-wide profiling of reactive cysteine ligandability remains a formidable task to adapt for high throughput applications. This is primarily due to...
ORGANISM(S): Homo sapiens (Human) 
2023-12-24 | PXD044402 | Pride
Reactive cysteine Abpp Chemoproteomics High throughput Tmt 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - HEK293T - Global expression proteomics
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. This platform profiles cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiobiotin (IA-...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054105 | Pride
Data independant acquistion Label free quantification 
ProteomicsReimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries
Current methods for measuring amino-acid side-chain reactivity lack the throughput needed to screen large chemical libraries for interactions across the proteome. Here we redesigned the workflow for activity-based protein profiling of reactive cysteine residues by using a smaller desthiobiotin-based...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-01-26 | PXD022511 | Pride
Reactive cysteines Chemical proteomics 
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