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We describe PROCAL (ProteomeTools Standard), a set of 40 synthetic peptides that span the entire hydrophobicity range of tryptic digests, enabling not only accurate determination of retention time indices but also monitoring of chromatographic separation performance over time. The fragmentation char...
ORGANISM(S): artificial sequences 
2017-09-07 | PXD006832 | Pride

BACKGROUND: Stool metabolites provide essential insights into the function of the gut microbiome. The current gold standard for storage of stool samples for metabolomics is flash-freezing at - 80 °C which can be inconvenient and expensive. Ambient temperature storage of stool is ...

2021-01-27 | MTBLS201 | MetaboLights
Genome duality in ciliated protozoa offers a unique system to showcase their epigenome as a model of inheritance. In Oxytricha, the somatic genome is responsible for vegetative growth, while the germline contributes DNA to the next sexual generation. Somatic nuclear development eliminates all transp...
ORGANISM(S): Sterkiella histriomuscorum 
Study question: Does storage time impact on transcriptome of slowly frozen cryopreserved human metaphase II (MII) oocytes? Summary answer: For the first time, we demonstrate that the length of cryostorage has no effect on the gene expression profile of human metaphase II oocytes. What is known alrea...
ORGANISM(S): Homo sapiens 
Pulmonary exposure to high doses of nanoparticles (NP) leads to well characterized lung toxicity in addition to long-term NP retention. However, pulmonary NP accumulation and toxicity following low dose exposures are not well described. In the present study we sought to: (1) investigate particle ret...
ORGANISM(S): Mus musculus 
All samples were processed by DIA individually to assess the proteome differences. MS1 and MS2 data were all acquired, and samples acquisition by random order. The iRT kit (Ki3002, Biognosys AG, Switzerland) was added to all of the samples to calibrate the retention time of extracted peptide peaks. ...
ORGANISM(S): Bos taurus (Bovine) 
2025-06-11 | PXD061007 | Pride
The retention of a nucleus in the mature state of fish red blood cells (RBCs), and the ability to easily collect and manipulate blood in non-terminal experiments, makes it an ideal tissue on which to study the cellular stress response in fish. Through the use of the cGRASP 16K salmonid microarray, w...
ORGANISM(S): Oncorhynchus mykiss 
We set out to investigate systematic changes in gene expression that may occur between surgical resection, routine processing in the histopathology department, frozen storage, and long term archiving in FFPE blocks. Lung carcinoma tissue samples were obtained from six patients during tumour resectio...
ORGANISM(S): Homo sapiens 
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