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ProteomicsDiverse roles of assembly factors revealed by structures of late nuclear pre-60S ribosomal particles
Ribosome biogenesis is a highly complex process in eukaryotes, involving temporally and spatially regulated ribosomal protein (r-protein) binding and rRNA remodeling events in the nucleolus, nucleoplasm and cytoplasm. Hundreds of assembly factors (AFs), organized into sequential functional groups, f...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-06-01 | PXD003736 | Pride
Assembly factors Cryo-em Pre-60s ribosome Cxms 
ProteomicsA comprehensive landscape of 60S ribosome biogenesis factors
We used quantitative proteomics and crosslinking mass spectrometry (XL-MS) to analyse a comprehensive set of purified pre-60S ribosomal particles, obtaining a detailed timeline of RBF engagement during 60S ribosome biogenesis as well as particle-specific crosslink networks for all analysed particles...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-12-07 | PXD021831 | Pride
Ribosome assembly factors (afs) Ribosome biogenesis factors (rbfs) Mi-filter False discovery rate (fdr) Crosslinking coupled to mass spectrometry (xl-ms) Ribosome biogenesis 
UnknownRibosome assembly factors prevent premature translation initiation by 40S assembly intermediates.
Not available
S-EPMC3402165 | biostudies-literature
OtherPol5 is required for recycling of small subunit biogenesis factors and for formation of the polypeptide exit tunnel of the large ribosomal subunit
More than 200 assembly factors (AFs) are required for the production of ribosomes in yeast. The stepwise association and dissociation of these AFs with the pre-ribosomal subunits occurs in a strictly hierarchical manner to ensure correct maturation of the pre-rRNAs and assembly of the ribosomal prot...
ORGANISM(S): Saccharomyces cerevisiae 
2019-11-05 | GSE132973 | GEO
ProteomicsRbp95 binds 25S rRNA helix H95 and cooperates with the Npa1 complex during early pre-60S particle maturation
Eukaryotic ribosome synthesis involves more than 200 assembly factors, which promote ribosomal RNA (rRNA) processing, modification and folding, and assembly of ribosomal proteins. The formation and maturation of the earliest pre-60S particles requires structural remodeling by the Npa1 complex, but i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-13 | PXD030106 | Pride
Ribosome assembly factor Yeast S. cerevisiae Tandem-affinity purification (tap) Npa1 Pre-60s particles Rbp95 
UnknownThe modifying enzyme Tsr3 establishes the hierarchy of Rio kinase binding in 40S ribosome assembly.
Not available
S-EPMC8925970 | biostudies-literature
UnknownStepwise assembly of the earliest precursors of large ribosomal subunits in yeast.
Not available
S-EPMC5499802 | biostudies-literature
UnknownThe ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events.
Not available
S-EPMC8266606 | biostudies-literature
ProteomicsEffect of Nop53 depletion on the compositional changes during pre-60S maturation
The eukaryotic ribosome biogenesis is a highly orchestrated multistep process that starts at the nucleolus with the transcription of pre-rRNAs 5S and 35S. The latter comprises the mature 18S, 5.8S and 25S rRNAs separated by internal transcribed spacers (ITS1 and ITS2) and externally flanked by the 5...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD023383 | Pride
Nop53 Eukaryotic ribosome biogenesis Pre-60s S. cerevisiae 
UnknownPol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit.
Not available
S-EPMC7145529 | biostudies-literature
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