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ProteomicsAptamer Affinity Mass Spectrometry
Antibody-based affinity purification of macromolecular complexes has revolutionized the study of protein-protein interactions. Here, we present AptA-MS (Aptamer Affinity – Mass Spectrometry), a robust strategy using a specific, high-affinity RNA aptamer against GFP to identify novel interactors of a...
ORGANISM(S): Homo sapiens (Human) 
2020-06-17 | PXD015620 | Pride
Hct116 Aptamer Rna aptamer Heat shock factor S2 
TranscriptomicsACE-Scan of the ATP RNA aptamer on 10^12 Gen1 DNA microarray
The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on ...
ORGANISM(S): synthetic construct 
2017-12-20 | E-MTAB-6376 | biostudies-arrayexpress
TranscriptomicsACE-Scan of the ATP DNA aptamer on various density Gen2 DNA microarrays
The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on ...
ORGANISM(S): synthetic construct 
2017-12-20 | E-MTAB-6375 | biostudies-arrayexpress
TranscriptomicsACE-Scan of the cocaine DNA aptamer on 10^12 Gen1 DNA microarray
The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on ...
ORGANISM(S): synthetic construct 
2017-12-20 | E-MTAB-6377 | biostudies-arrayexpress
TranscriptomicsACE-Scan of the ATP DNA aptamer on 10^12 density Gen1 DNA microarray
The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on ...
ORGANISM(S): synthetic construct 
2017-12-20 | E-MTAB-6374 | biostudies-arrayexpress
TranscriptomicsACE-Scan of the natural add riboswitch aptamer on 10^10 Gen1 DNA microarrays with varying buffer temperatures and Mg2+ cation concentrations
The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on ...
ORGANISM(S): synthetic construct 
2017-12-20 | E-MTAB-6380 | biostudies-arrayexpress
TranscriptomicsACE-Scan of the thrombin DNA aptamer on 10^10 Gen1 DNA microarrays with sodium and potassium buffers in two colors.
The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on ...
ORGANISM(S): synthetic construct 
2017-12-20 | E-MTAB-6378 | biostudies-arrayexpress
MetabolomicsIdentification of the modifications in artificial E. coli rRNAs
Ribosome biogenesis is pivotal in the self-replication of life. In Escherichia coli, three ribosomal RNAs and 54 ribosomal proteins are synthesized and subjected to cooperative hierarchical assembly facilitated by numerous accessory factors. Realizing ribosome biogenesis in vitro is a critical miles...
2024-12-09 | MTBLS7425 | MetaboLights
UnknownGluK2 kainate receptor subunit-selective, potentiating RNA aptamer.
Not available
S-EPMC12426214 | biostudies-literature
ProteomicsDifferential RNA aptamer affinity profiling on plasma as a potential diagnostic tool for bladder cancer
APTASHAPE is a new method for characterising the composition of complex biofluids Biomarker discovery is performed using blood from bladder cancer patients APTASHAPE can distinguish blood samples from patients with different stages of bladder cancer
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD032972 | Pride
Pulldown Biofluids Aptamer Bladder cancer 
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