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ProteomicsPuf3 Tap Tag and Mass Spectrometry in Not4 Delta and WT Yeast Strains
Investigation of the proteins associated with Puf3, an RNA-binding protein, in Saccharomyces cerevisiae. The goal is to compare the Puf3 interactome in a wild-type strain and in a Not4-deleted strain (Δnot4), in order to assess the potential role of Not4 in modulating Puf3-associated complexes. Puf3...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-09-14 | PXD069853 | Pride
Yeast puf3 tap tag not4 deletion wt ap mass spectrometry yeast 
ProteomicsInterrogating the interaction of fission yeast RNA-binding protein Puf3 with the Ccr4-Not complex using crosslinking mass spectrometry
mRNA decay is a key determinant of gene regulation, initiated by the shortening of mRNA poly(A) tails by the CCR4-NOT deadenylation complex. Specificity is achieved through RNA adaptors—RNA-binding proteins that recruit CCR4-NOT to specific substrate mRNAs in a regulated manner. However, the molecul...
ORGANISM(S): Schizosaccharomyces pombe 927 
2025-09-03 | PXD055147 | Pride
Crosslinking ms Idr Protein-protein interaction Rna binding protein puf3 Ccr4-not complex 
TranscriptomicsLooking for mitochondrial associated RNA
We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR) in various conditions in which translation was inhibited or the mRNA binding protein context altered (Delta puf3). We used cell fractionation protocols together with microarray to assess the distribution of mRNA...
ORGANISM(S): Saccharomyces cerevisiae 
2008-06-01 | E-GEOD-9393 | biostudies-arrayexpress
ProteomicsSchizosaccharomyces pombe Puf3 interactome
Puf3 is a RNA-binding protein, a member of the conserved Puf-protein family. Combining different functional genomics data, we have analyzed the role of Puf3 in post-transcriptional gene regulation in S. pombe. We present data on Puf3 interacting proteins and regulatory mRNA targets.
ORGANISM(S): Schizosaccharomyces pombe 972h- 
2022-03-23 | PXD006318 | Pride
Rip-chip Puf3 Tap purification Protein interactors Rna-binding protein Post-transcriptional regulation 
UnknownPUF3 RNA binding protein of Trypanosoma cruzi regulates mitochondrial morphology and function.
Not available
S-EPMC11252720 | biostudies-literature
TranscriptomicsCoordinating Expression of RNA Binding Proteins with Their mRNA Targets
Post-transcriptional regulation by RNA binding proteins (RBPs) plays prominent roles in a variety of biological processes. In this study, by analyzing the global regulatory relationship between RBPs and their target mRNAs in yeast, we discovered that most RBP genes are co-regulated with their target...
ORGANISM(S): Saccharomyces cerevisiae 
2014-06-01 | E-GEOD-55419 | biostudies-arrayexpress
UnknownRoles of the Pumilio domain protein PUF3 in Trypanosoma brucei growth and differentiation.
Not available
S-EPMC10317725 | biostudies-literature
UnknownRpb4 and Puf3 imprint and post-transcriptionally control the stability of a common set of mRNAs in yeast.
Not available
S-EPMC8244751 | biostudies-literature
GenomicsPuf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4
Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4
PRJNA1307569 | ENA
OtherPuf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4
Here, we describe that Not4 and Puf3 interact to regulate protein homeostasis. mRNAs less soluble upon Not4 depletion but more upon Not1 depletion are enriched for targets of the yeast Puf3 RNA binding protein.
ORGANISM(S): Saccharomyces cerevisiae 
2026-08-26 | GSE305765 | GEO
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