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This study aimed to identify protein interaction partners of the lncRNA vA-202 using an RNA pull-down approach followed by mass spectrometry. Biotinylated vA-202 RNA was generated by in vitro transcription incorporating biotin-14-CTP. For each pull-down reaction, 400 µg of whole-cell lysate (in a to...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD070150 | Pride
mir-154 in mouse lung development - pull-down RNA
Long non-coding RNAs (lncRNAs) play critical regulatory roles in primate-specific biological processes, yet many primate-specific lncRNAs and their protein interactors remain poorly characterized. In this study, we focused on primate-specific lncRNAs as potential drivers of protein interactions. Usi...
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD075354 | Pride
ACSL4mRNA probe was designed for pull-down experiments in HaCaT lysates
ORGANISM(S): Homo sapiens (Human) 
2025-10-13 | PXD052755 | Pride
RNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
Antisense and sense probes of noncoding RNA GAS5 were used to perform the RNA pull down experiment on mouse hippocampus tissues. Then the proteins were identified by LC-MS/MS.
ORGANISM(S): Mus musculus (Mouse) 
2021-09-09 | PXD023363 | Pride
To screen Snhg3-binding proteins using-RNA Pull down and MS
ORGANISM(S): Mus Musculus 
2023-01-17 | PXD039526 |
RNA pull-down assay.
For the recombinant protein pull-down assays, 50 M-5g of recombinant His-tag TcRBP40 protein were bound to 100 uL of Ni-NTA resin (Qiagen) overnight at 4M-0C. 100 M-5g of total RNA from epimastigotes were incubated with the bound protein in 500 M-5l EMSA buffer at 4M-0C for 2...
ORGANISM(S): Trypanosoma cruzi 
Long noncoding RNAs (lncRNAs) have emerged as important components of gene regulatory network in embryonic stem cells (ESCs). However, the function and molecular mechanism of lncRNAs are still largely unknown. Here we identified Trincr1 (TRIM71 interacting long noncoding RNA 1) lncRNA that regulates...
ORGANISM(S): Mus musculus (Mouse) 
2019-02-01 | PXD012493 | Pride
RNA pull-down analysis of hsa_circ_0001400 in 293T cells
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