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ProteomicsRNAPII interactome via RPB3-FLAG IP
FLAG Ab pull down of chromatin associated proteins in the stable cell line expressing RPB3-3xFLAG (with DOX). Total RNAPII interactome was measured in three conditions; Untreated, 1mM H2O2 treatment for 15 minutes, and 1 hour recovery following media washout.
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD067831 | Pride
Total rnapii Rpb3 Flag 
TranscriptomicsGenome-associated RNA Polymerase II includes the dissociable Rpb4/7 subcomplex
Yeast RNA polymerase (Pol) II consists of a ten-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immuno-precipitation coupled to h...
ORGANISM(S): Saccharomyces cerevisiae 
2008-07-29 | E-GEOD-12060 | biostudies-arrayexpress
UnknownRpb3 promotes hepatocellular carcinoma through its N-terminus.
Not available
S-EPMC4253432 | biostudies-literature
GenomicsPolII Rpb3 ChIP-seq data from D. melanogaster
PolII Rpb3 ChIP-seq data from D. melanogaster
PRJEB3031 | ENA
ProteomicsPol II preferentially regulates ribosomal protein expression by trapping disassociated subunits
Pol II has been recognized as a passively regulated holoenzyme. However, whether Pol II plays specific regulatory roles remain unclear. Here, fractions containing disassociated RPB3 (dRPB3) were identified by size exclusion chromatography in various cells. Through a unique strategy, Specific Degrada...
ORGANISM(S): Homo sapiens (Human) 
2023-02-14 | PXD038474 | Pride
Hek293t rpb3 ptm 
UnknownDistinct regions of RPB11 are required for heterodimerization with RPB3 in human and yeast RNA polymerase II.
Not available
S-EPMC1159119 | biostudies-literature
GenomicsWhole genome recruitment of Rpb3 and Rpb4 in S.cerevesiae
ChIP on chip microarray experiment to decipher Rpb3 and Rpb4 recruitment in S.cerevesiae genome. Keywords: ChIP-chip
ORGANISM(S): Saccharomyces cerevisiae 
2007-11-15 | GSE9265 | GEO
GenomicsGenome-wide nucleosome, histone and Pol II maps for wild type Saccharomyces cerevisiae induced with 3-aminotriazole (3AT)
Paired-end sequencing study of (1) nucleosome core particles and under-digested chromatin from MNase-treated nuclei; (2) ChIP samples for HA-tagged histone H4 and H2B; (3) ChIP for the Rpb3 subunit of Pol II. Nucleosomal DNA and immunopurified sonicated DNA fragments were subjected to paired-end seq...
ORGANISM(S): Saccharomyces cerevisiae 
2014-10-28 | E-GEOD-54524 | biostudies-arrayexpress
UnknownActivation mutants in yeast RNA polymerase II subunit RPB3 provide evidence for a structurally conserved surface required for activation in eukaryotes and bacteria.
Not available
S-EPMC316356 | biostudies-literature
GenomicsComparison of GRO-seq with Pol II (Rpb3) ChIP-seq in Drosophila melanogaster S2 cells
Comparison of GRO-seq with Pol II (Rpb3) ChIP-seq in Drosophila melanogaster S2 cells
PRJNA131825 | ENA
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