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ProteomicsCombining transcriptome and proteome analysis to identify and characterize the RpoS regulon in Pseudomonas fluorescens
Background: Microorganisms are the major cause of food spoilage during storage, processing and distribution. Pseudomonas fluorescens is a typical spoilage bacterium that contributes to a large extent to the spoilage process of proteinaceous food. RpoS is considered an important global regulator invo...
ORGANISM(S): Pseudomonas fluorescens 
2019-11-12 | PXD010845 | Pride
Transcriptome Pseudomonas fluorescens Rpos Proteome Regulon 
TranscriptomicsComparison between Borrelia burgdorferi wild type strain and cognate rpoS, rpoN and rrp2 mutants
The RpoN-RpoS alternative sigma factor pathway is essential for key adaptive responses by Borrelia burgdorferi, particularly those involved in the infection of a mammalian host. A putative response regulator, Rrp2, ostensibly is required for activation of the RpoN-dependent transcription of rpoS. ...
ORGANISM(S): Borrelia burgdorferi 
2008-09-09 | E-GEOD-11284 | biostudies-arrayexpress
TranscriptomicsTranscriptome of Legionella pneumophila rpoS mutant in water
Legionella pneumophila is a water-borne pathogen, and thus survival in the aquatic environment is central to its transmission to humans. Hence, identifying genes required for its survival in water could help prevent Legionnaires’ disease outbreaks. In the present study, we investigate for the first ...
ORGANISM(S): Legionella pneumophila subsp. pneumophila str. Philadelphia 1 
2014-12-04 | E-GEOD-56258 | biostudies-arrayexpress
TranscriptomicsEvolution of E. coli transcriptome under osmotic-stress with and without RpoS
The RpoS sigma factor protein of Escherichia coli is the master transcriptional regulator of physiological responses to a variety of stresses. This stress response comes at the expense of scavenging for scarce resources, causing a trade-off between stress tolerance and nutrient acquisition. This t...
ORGANISM(S): Escherichia coli 
2009-11-09 | E-GEOD-13666 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E.coli wild type vs RpoS mutants during exponenitail growth
This study is to identify the RpoS regulon in MG1655 in early exponential growth. RNA samples from wild type or rpoS mutants were extracted using acidic hot phenol method and hybridized to Affymetrix E. coli Antisense Genome Array. Experiment Overall Design: A precise rpoS deletion mutant of MG1655 ...
ORGANISM(S): Escherichia coli K12 
2008-06-16 | E-GEOD-9814 | biostudies-arrayexpress
TranscriptomicsGlobal effect of RpoS on gene expression in pathogenic Escherichia coli O157:H7 strain EDL933
RpoS is a conserved stress regulator that plays a critical role in survival under stress conditions in Escherichia coli and other γ-proteobacteria. RpoS is also involved in virulence of many pathogens including Salmonella and Vibrio species. Though well characterized in non-pathogenic E. coli K12 st...
ORGANISM(S): Escherichia coli O157:H7 EDL933 
2009-08-03 | E-GEOD-17420 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Vibrio cholerae wild type, hapR and rpoS deletions in mid-exponential or stationary phase concluding that rpoS controls the Vibrio cholerae mucosal escape response
For expression analysis of wild-type V. cholerae, hapR, and rpoS deletion mutants in mid-exponential or stationary phase, the strains were grown to either OD600 of 0.3 or for 11 h in LB media at 37 0C, and bacteria from 2-ml culture were quickly pelleted, resuspended in Trizol reagent (GIBCO/BRL, Sa...
ORGANISM(S): Vibrio cholerae 
2006-11-06 | E-SMDB-3833 | biostudies-arrayexpress
TranscriptomicsBorrelia host adaptation Regulator (BadR) regulates rpoS to modulate host adaptation and virulence factors in Borrelia burgdorferi
Our study is the first to demonstrate BadR as a repressor of rpoS and thus facilitates spirocheteM-bM-^@M-^Ys transition back into ticks. BadR binds upstream and represses rpoS in unfed ticks. GLcNA-6P, an abundant nutrient of blood and released by subsequent remodeling of the tick peritrophic memb...
ORGANISM(S): Borrelia burgdorferi B31 
2013-02-22 | E-GEOD-38827 | biostudies-arrayexpress
GenomicsSerratia marcescens strain:RPOS 1039
Serratia marcescens strain:RPOS 1039 Genome sequencing
PRJNA1424210 | ENA
GenomicsChIP-seq of Escherichia coli RpoS during stationary phase
ChIP-seq of Escherichia coli RpoS during stationary phase
PRJEB18602 | ENA
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