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ProteomicsHPLC-TIMS-TOF-MS of purified mitochondrial I+III2 supercomplex from Arabidopsis thaliana
Mitochondrial I+III2 supercomplex was purified from Arabidopsis thaliana cells using differential centrifugation including Percoll step gradients and continuous sucrose gradients. Supercomplex fractions were digested with trypsin using the SP3 approach. Peptides were further purified with SepPak car...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-12-30 | PXD036482 | Pride
Arabidopsis Supercomplex Mitochondria 
ProteomicsMulti-Protease Investigation of Arabidopsis Thaliana Cell Culture Mitochondria
The aim of this project is to deeply map the proteome of mitochondria from the model plant Arabidopsis thaliana. For this purpose, mitochondria were isolated from Arabidopsis cell cultures, their proteins extracted and processed using SP3 digestion. To achieve high sequence coverage, the proteins we...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2023-08-02 | MSV000092578 | MassIVE
ProteomicsProteomics Analysis of the UMK2 Mutation in Arabidopsis thaliana seeds
We compared the proteome of dry as well as germinating seeds of Arabidopsis thaliana wild type Col-0 with the respective proteomes from an umk2 (AT4G25280) frameshift mutant. Germinating seeds were incubated for 48 hours in 1/2-MS medium in Petri dishes at 4 degree Celsius followed by 24 hours of a...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2023-07-03 | MSV000092331 | MassIVE
ProteomicsProteomic investigation of seed development under high nitrate supply in plants lacking Pyrroline-5-carboxylate dehydrogenase
Free proline is an amino acid known to have many functions in plants, particularly in stress adaptation and development. Its metabolism is well known, but its exact role in plant physiology has remained elusive. Proline catabolism is ensured by the sequential action of the enzymes Proline dehydrogen...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-06-10 | MSV000094979 | MassIVE
ProteomicsAVATARS - Tissue-specific multi-omics analyses in developing Brassica napus seeds [proteomics]
Seeds of the German winter-type oilseed rape cultivar Express 617 were sampled and analysed at five key stages of seed development. Express 617 is a high oil yield cultivar with double-zero quality (low erucic acid and low glucosinolate content), which has been widely used in breeding programs and a...
ORGANISM(S): Brassica napus (Rape) 
2025-03-19 | PXD053532 | Pride
Seed filling Seed coat Rapeseed Brassica napus Seeds  seed development Cotyledons Radicle 
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