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ProteomicsPacific Ocean metaproteomics, METZYME KM1128
Global metaproteomic analyses of microbial biomass from the upper water column of the Central Pacific Ocean. This dataset was used as a discovery dataset to identify peptide biomarkers for cyanobacterial populations for use in targeted metaproteomic calibrated multiple reaction monitoring (MRM) anal...
ORGANISM(S): Environmental Samples (ncbitaxon:318178) 
2021-11-05 | MSV000088323 | MassIVE
ProteomicsDinoflagellates contribute to primary and secondary production across biogeochemical gradients of the central Pacific Ocean
In this study we explored the metabolism of unicellular eukaryotic organisms (protists) across a 4,600 km meridional transect in the central Pacific Ocean. The region contains a natural biogeochemical gradient spanning from low nitrogen, oligotrophic waters to a productive equatorial upwelling syste...
ORGANISM(S): Phytoplankton Environmental Sample (ncbitaxon:1249885) 
2021-11-03 | MSV000088314 | MassIVE
ProteomicsLack of a Zn/Co substitution ability in the polar diatom Chaetoceros RS19
Functional substitution of the essential trace metals zinc (Zn) and cobalt (Co) within metalloenzymes has been well documented in marine diatoms and is known to be prevalent among varying genera and species. In contrast to the majority of species studied to date, we find that the polar diatom Chaeto...
ORGANISM(S): Chaetoceros sp. 
2022-03-02 | PXD026895 | Pride
Chaetoceros Cobalt Diatom Zinc 
ProteomicsTwo novel zinc and cobalt-responsive proteins in marine eukaryotic phytoplankton and implications for Zn scarcity in the surface oceans
Observations of scarce surface ocean concentrations of dissolved zinc (Zn) have raised the possibility that Zn may co-limit the growth of oceanic phytoplankton, yet testing this hypothesis has been difficult because Zn is highly prone to contamination. Biomarkers of micro-nutrient stress can serve a...
ORGANISM(S): Thalassiosira pseudonana CCMP1335 Phaeodactylum tricornutum (strain CCAP 1055/1) Pseudo-nitzschia delicatissima Chaetoceros sp. 
2022-03-02 | PXD026953 | Pride
Cobalt Proteomics Diatoms Pseudo nitzschia Thalassiosira pseudonana Chaetoceros Zinc 
ProteomicsTranscriptomic and proteomic responses of the oceanic diatom Pseudo-nitzschia granii to iron limitation
The oceanic diatom Pseudo-nitzschia granii was cultured in the laboratory under steady-state iron-replete and iron-limited conditions. Transcriptomic and proteomic analyses were performed to determine how this organism reorganizes major metabolic processes as a function of iron supply.
ORGANISM(S): Pseudo-nitzschia granii 
2018-08-21 | PXD009698 | Pride
Iron limitation Diatom 
ProteomicsOnline Nanoflow Two-Dimension Comprehensive Active Modulation Reversed Phase-Reversed Phase Liquid Chromatography High Resolution Mass Spectrometry for Metaproteomics of Environmen-tal and Microbiome Samples
Metaproteomics is a powerful analytical approach that can assess the functional capabilities deployed by microbial communi-ties in both environmental and biomedical microbiome settings. Yet the mass spectra resulting from these mixed biological communities are challenging due to the high number of l...
ORGANISM(S): Homo sapiens (Human) marine plankton environmental sample 
2022-05-20 | PXD027351 | Pride
Automated Metaproteomics Proteomics Comprehensive active modulation 2dlc Rprp Lc Ocean Lcms 
ProteomicsFlexible B12 ecophysiology of Phaeocystis antarctica due to a B12-independent methionine synthase fusion protein with widespread homologues
Coastal Antarctic marine ecosystems play an important role in carbon cycling due to their highly productive seasonal phytoplankton blooms. Southern Ocean microbes are primarily limited by light and iron (Fe) and can be co-limited by cobalamin (vitamin B12 ). Micronutrient limitation is a key driver ...
ORGANISM(S): Phaeocystis antarctica 
2023-02-27 | PXD031524 | Pride
Lc-msms Vitamin b12 Phaeocystis antarctica Methionine synthase fusion protein 
ProteomicsDinoflagellates contribute to primary and secondary production across biogeochemical gradients of the central Pacific Ocean
In this study we explored the metabolism of unicellular eukaryotic organisms (protists) across a 4,600 km meridional transect in the central Pacific Ocean. The region contains a natural biogeochemical gradient spanning from low nitrogen, oligotrophic waters to a productive equatorial upwelling syste...
ORGANISM(S): phytoplankton environmental sample 
2020-09-18 | PXD014230 | Pride
Phytoplankton Pacific ocean Dinoflagellates 
ProteomicsClimate sensitivity of Equatorial Pacific nutrient limitation
Projected responses of ocean net primary productivity (NPP) to climate change are highly uncertain. The climate sensitivity of phytoplankton nutrient limitation in the low-latitude Pacific plays a crucial role, but field measurements are insufficient to provide suitable constraints. Here we quantify...
ORGANISM(S): marine plankton environmental sample 
2023-06-26 | PXD030610 | Pride
Metaproteomics Phytoplankton Chlorophyl Iron limitation Prochlorococcus Ocean 
ProteomicsOcean warming enhances iron use efficiencies of marine ammonia-oxidizing archaea
Ammonia-oxidizing archaea (AOA) are among the most abundant microorganisms in the ocean, playing a fundamental role in the marine nitrogen cycle. Although key environmental variables such as temperature and trace metal availability are known to influence the growth and activity of marine AOA, there ...
ORGANISM(S): Nitrosopumilus maritimus SCM1 Archaea 
2026-09-14 | PXD049308 | Pride
Nitrosopumlus maritimus scm1 Aoa Ammonia oxidizing archaea Lcmsms 
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