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ProteomicsPacific Ocean metaproteomics, METZYME KM1128
Global metaproteomic analyses of microbial biomass from the upper water column of the Central Pacific Ocean. This dataset was used as a discovery dataset to identify peptide biomarkers for cyanobacterial populations for use in targeted metaproteomic calibrated multiple reaction monitoring (MRM) anal...
ORGANISM(S): Environmental Samples (ncbitaxon:318178) 
2021-11-05 | MSV000088323 | MassIVE
ProteomicsDinoflagellates contribute to primary and secondary production across biogeochemical gradients of the central Pacific Ocean
In this study we explored the metabolism of unicellular eukaryotic organisms (protists) across a 4,600 km meridional transect in the central Pacific Ocean. The region contains a natural biogeochemical gradient spanning from low nitrogen, oligotrophic waters to a productive equatorial upwelling syste...
ORGANISM(S): Phytoplankton Environmental Sample (ncbitaxon:1249885) 
2021-11-03 | MSV000088314 | MassIVE
ProteomicsTemperature (20-28ºC) and nutrient stress (N- and P-stress) on Synechococcus WH8102
Cultures of Synechococcus (WH8102) were grown in polycarbonate bottles with a continuous culturing method with an artificial seawater medium. The experimental design included two concentration ratios of macronutrients (NO3-:PO43- = 1.7 and 80) and 3 levels of temperature (20, 24 and 28°C). Light was...
ORGANISM(S): Bacteria Synechococcus sp. WH 8012 
2024-06-16 | PXD043180 | Pride
Synechococcus wh8102 Environmental Lcmsms 
ProteomicsOnline Nanoflow Two-Dimension Comprehensive Active Modulation Reversed Phase-Reversed Phase Liquid Chromatography High Resolution Mass Spectrometry for Metaproteomics of Environmen-tal and Microbiome Samples
Metaproteomics is a powerful analytical approach that can assess the functional capabilities deployed by microbial communi-ties in both environmental and biomedical microbiome settings. Yet the mass spectra resulting from these mixed biological communities are challenging due to the high number of l...
ORGANISM(S): Homo sapiens (Human) marine plankton environmental sample 
2022-05-20 | PXD027351 | Pride
Automated Metaproteomics Proteomics Comprehensive active modulation 2dlc Rprp Lc Ocean Lcms 
ProteomicsLack of a Zn/Co substitution ability in the polar diatom Chaetoceros RS19
Functional substitution of the essential trace metals zinc (Zn) and cobalt (Co) within metalloenzymes has been well documented in marine diatoms and is known to be prevalent among varying genera and species. In contrast to the majority of species studied to date, we find that the polar diatom Chaeto...
ORGANISM(S): Chaetoceros sp. 
2022-03-02 | PXD026895 | Pride
Chaetoceros Cobalt Diatom Zinc 
ProteomicsDinoflagellates contribute to primary and secondary production across biogeochemical gradients of the central Pacific Ocean
In this study we explored the metabolism of unicellular eukaryotic organisms (protists) across a 4,600 km meridional transect in the central Pacific Ocean. The region contains a natural biogeochemical gradient spanning from low nitrogen, oligotrophic waters to a productive equatorial upwelling syste...
ORGANISM(S): phytoplankton environmental sample 
2020-09-18 | PXD014230 | Pride
Phytoplankton Pacific ocean Dinoflagellates 
ProteomicsThe ProteOMZ Expedition: Investigating Life Without Oxygen in the Pacific Ocean
Untargeted proteomics from a 5,000 km+ transect across the central Pacific Ocean from Hawaii to Tahiti. The expedition crossed multiple biogeochemical provinces, inlcuding the oligotrophic North Pacific Subtropical Gyre, the extremety of the Eastern Tropical North Pacific Oxygen Deficient Zone, and ...
ORGANISM(S): marine metagenome 
2022-08-31 | PXD030684 | Pride
Microbial ecology Mesopelagic Oxygen minimum zone Equatorial upwelling Oxygen deficient zone Nitrification Ocean Marine Pelagic 
ProteomicsPhotoacclimation and functional control of stolen organelles in Mesodinium rubrum by an enslaved nucleus
The enslavement of foreign organelles by protists for metabolic gain is a common phenomenon within aquatic ecosystems. Ciliates belonging to the Mesodinium rubrum species complex are unique in that they also steal a transcriptionally active prey nucleus, the kleptokaryon, from certain cryptophytes, ...
ORGANISM(S): Mesodinium rubrum 
2025-01-07 | PXD035728 | Pride
Lc-msms Marine ciliate Mesodinium rubrum Phototrophy 
ProteomicsProteomics analysis reveals differential acclimation of coastal and oceanic Synechococcus to climate warming and iron limitation
In many regions of the ocean, anthropogenic warming will coincide with iron (Fe) limitation. Interactive effects between warming and Fe limitation on phytoplankton physiology and biochemical function are likely, as temperature and Fe availability affect many of the same essential cellular pathways. ...
ORGANISM(S): Escherichia coli 
2025-09-29 | PXD045922 | Pride
Marine Iron limitation Synechococcus Lcms 
ProteomicsA proteomic perspective on homeostasis mechanisms and broader cellular effects of zinc on model prokaryote Pseudomonas aeruginosa
Zinc is central to the function of many proteins, yet the mechanisms of zinc homeostasis and its impact on other cellular regulatory systems remain under-characterized. In this study, we used the opportunistic human pathogen and environmentally significant model organism Pseudomonas aeruginosa to ex...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2026-08-31 | PXD047454 | Pride
Lc-msms Pseudopaline Abc transporter Pseudomonas aeruginosa Biofilm Zinc Metalloprotein 
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