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ProteomicsInteractome of S65 phosphorylated NEDD8 and Ub
In this project we present for the first time a proteome-wide interaction study of S65 phosphorylated NEDD8 and ubiquitin (Ub). By using cell extracts and affinity enrichment coupled to mass spectrometry, we show that pNEDD8 and pUb have different interactomes that are also distinct from those of th...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD021143 | Pride
Phosphorylation Nedd8 Ubiquitin Interactome 
ProteomicsNEDD8 S65 phosphorylation under different stress conditions
In this project we present the identification of NEDD8 S65 phosphorylation under mitochondrial and DNA double strand break (DSB) stress conditions. Relative comparison revealed elevated levels of pNEDD8 under mitochondrial stress and reduced levels of pNEDD8 under DSB stress.
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD027477 | Pride
Stress Phosphorylation Nedd8 
TranscriptomicsRNAi knock down of HectH9 in human osteosarcoma samples to identify myc regulated genes
Analysis of Gene Regulation by Myc in the absence of HectH9
ORGANISM(S): Homo sapiens 
2005-10-17 | E-MEXP-406 | biostudies-arrayexpress
Proteomicsp300 acetylates Ub and linear Ub dimers
In this project we present the identification of in vitro acetylation of Ub by the acetyltransferase p300. Free monomeric Ub or M1-linked linear Ub dimers (diUb) were incubated in vitro with p300 in presence or absence of acetyl-Coenzyme A (AcCoA). For identification of the acetylation status of the...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD028813 | Pride
Acetylation Acetyltransferase p300 Ub 
TranscriptomicsTranscription profiling of two mouse myc mutants Eilers-Mouse-MycKR6 vs Mycwt
Comparison gene regulation by two different Myc mutants
ORGANISM(S): Mus musculus 
2005-10-17 | E-MEXP-407 | biostudies-arrayexpress
ProteomicsIdentification of interactors of acetylated Ub
In this project we present the identification of interactors of mono-acetylated ubiquitin (Ub) variants from HEK293T whole cell extracts. Affinity enrichment coupled to high-resolution mass spectrometry followed by label-free quantification revealed the interactome of the respective mono-acetylated ...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD028797 | Pride
Acetylation Ub Interactome 
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