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Wallerian degeneration (WD) involves the fragmentation of axonal segments disconnected from their cell bodies, segmentation of the myelin sheath, and removal of debris by Schwann cells and immune cells. The removal and downregulation of myelin-associated inhibitors of axonal regeneration and synthes...
ORGANISM(S): Mus musculus 
profiles of normal and diseased sciatic nerve of young adult mice Keywords: other
ORGANISM(S): Mus musculus 
We applied Solexa sequencing technology to identify rat microRNA genes in proximal sciatic nerve following sciatic nerve resection. Using Solexa sequencing, computational analysis and Q-PCR verification, 93 novel miRNAs in rats were discovered and identified, of which 42 novel miRNAs were first repo...
ORGANISM(S): Rattus norvegicus 
wild-type mouse (FVB strain) expression profiles of adult sciatic nerve and of cultured Schwann cell Keywords: other
ORGANISM(S): Mus musculus 
We used microarrays to distinguish the gene expression differences among different time points after injury. We generated proximal sciatic nerve (SN) tissues (0.5cm) at 0h, 0.5h, 1h, 3h, 6h and 9h after sciatic nerve resection.
ORGANISM(S): Rattus norvegicus 
Lysine acetylation is a reversible Post-translational modification (PTM) involved in a broad array of physiological functions. Recent studies have demonstrated the involvement of protein acetylation in modulating Schwann cells' (SCs) biology and the peripheral nervous system (PNS) regeneration. Howe...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-15 | PXD029611 | Pride
ChIP-seq of H3K27ac in P15 sciatic nerve 1 H3K27ac ChIP sample, with respective input
ORGANISM(S): Rattus norvegicus 
As rats do not develop neuropathic pain like hypersensitivity as neonates post nerve injury but do as adults we have used these arrays to help define the processes involved in this process. Rat spinal cord (ipsilateral dorsal horn) was assayed 7 days post SNI injury to the sciatic nerve relative to ...
ORGANISM(S): Rattus norvegicus 
The NuRD complex is required for efficient and timely myelination in the peripheral nervous system. ChIP-chip assays were performed on rat sciatic nerve at P15, a peak timepoint of myelination, for binding of Chd4 to genes involved in regulating myelin formation. This experiment includes two custom...
ORGANISM(S): Rattus norvegicus 
The age and sex of studied animals profoundly impacts experimental outcomes in animal-based biomedical research. However, most preclinical studies in mice use a wide-spanning age range from 4 to 14 weeks and do not assess study parameters in male and female mice in parallel. This raises concerns reg...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-29 | PXD034476 | Pride
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