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ProteomicsiTRAQ MS of mouse LbetaT2 cells transfected with Galphas siRNA vs. scrambled siRNA
Twenty million LbetaT2 cells were transfected with either control or Galphas siRNA, then were seeded in 100-mm cell culture plates in DMEM + 10% FBS. Two days later, cells were washed twice with pre-warmed PBS. Conditioned media was harvested another 24 h later, and centrifuged at 20,000 g for 10 mi...
ORGANISM(S): Mus musculus (Mouse) 
2016-09-27 | PXD000063 | Pride
Pituitary gonadotrope-derived lbetat2 cells Ab sciex tof/tof 5800 Mouse 
ProteomicsProteome profiling of three cell populations from the common starfish Asterias rubens
The origin of cells involved in regeneration in Echinoderms is an open question till now. Most experimental data support the process of transdifferentiation as the main mechanism, although the activity of progenitor/stem cells is also considered. The renewal of coelomocytes, the cells which occupy t...
ORGANISM(S): Asterias rubens 
2020-12-09 | PXD012550 | Pride
Coelomic epithelium Progenitor cells Asterias rubens Lc-maldi Coelomocytes Sciex 5800 
UnknownProduction and secretion dynamics of prokaryotic Penicillin G acylase in Pichia pastoris.
Not available
S-EPMC7306039 | biostudies-literature
ProteomicsMultifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration
With the use of iTRAQ technique, a multifactorial comparative proteomic study can be performed. In this study, to obtain an overview of ethanol, CYP2E1 and gender effects on liver injury and gain more insight into the underlying molecular mechanism, mouse liver proteomes were quantitatively analyze...
ORGANISM(S): Mus musculus (Mouse) 
2014-04-11 | PXD000635 | Pride
Alcohol metabolism Liver Cyp2e1 Multifactorial comparative proteome Itraq 
ProteomicsA novel domain essential for interdomain cooperativity in binding of a Rep protein to DNA.
A very important feature of replication initiation proteins is the ability to bind to DNA via a characteristic domain. Usually, one or two DNA-binding domains can be distinguished within each initiator. In this work, we specified a new family of replication initiation proteins (the TrfA-like protein...
ORGANISM(S): Escherichia coli 
2021-09-08 | PXD013286 | Pride
Dna replication initiation Trfa Rep proteins Mass spectrometry Dna binding domain Winged helix domain Plasmid rk2 
ProteomicsProteome profile analysis of boron-induced alleviation of aluminum-toxicity in Citrus grandis roots by 2-dimentional electrophoresis (2-DE) technique
After long-term different boron and aluminum treatment, 2D electrophoresis and mass spectrum were conducted to investigate different proteiomic profile in Citrus grandis roots samples.
ORGANISM(S): Citrus maxima (Pomelo) (Citrus grandis) 
2019-01-28 | PXD006765 | Pride
Aluminum Boron 2-de Proteomic Citrus grandis Alleviation 
ProteomicsBoron-toxicity-induced alterations of protein profiles in roots of two citrus species differing in boron-tolerance revealed by 2-DE
After long-term different boron and aluminum treatment, 2D electrophoresis and mass spectrum were conducted to investigate different proteiomic profile in Citrus sinensis roots samples.
ORGANISM(S): Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis) 
2018-10-26 | PXD004050 | Pride
Boron-toxicity • citrus grandis • citrus sinensis • 2-de • proteome • roots 
ProteomicsProteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency
After long-term magnesium-deficiency treatment, 2D electrophoresis and mass spectrum were conducted to investigate different proteiomic profile in Citrus sinensis roots and leaves samples.
ORGANISM(S): Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis) 
2015-04-13 | PXD001871 | Pride
Citrus sinensis Proteomic analysis 
ProteomicsLow pH-responsive proteins revealed by a 2-DE based MS approach and related physiological responses in Citrus leaves
After long-term low pH treatment, 2D electrophoresis and mass spectrum were conducted to investigate different proteiomic profile in Citrus sinensis and Citrus grandis leaves samples.
ORGANISM(S): Citrus maxima (Pomelo) (Citrus grandis) 
2018-10-16 | PXD010426 | Pride
Citrus grandis Citrus sinensis 2-de Leaves Proteomics Low ph 
Proteomics2D-DIGE global protein profiling of alcohol-dependent mouse brain
Data analysis. Spot detection and matching were performed with a comparative cross analysis of all the gels using DeCyder software v.6.5 (GE Healthcare). 178 spots were selected based on 1.15-fold for protein ratio cut-off, allowing for the appearance of the spots in 23 out of 28 gels (69 out of 8...
ORGANISM(S): Mus musculus (Mouse) 
2014-07-28 | PXD000349 | Pride
Cie Mouse Brain Alcohol dependence Midbrain Cortex 2d-dige 
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