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MetabolomicsA Cross-Platform Metabolomics Comparison Identifies Serum Metabolite Signatures of Liver Fibrosis Progression in Chronic Hepatitis C Patients
Metabolomics offers new insights into disease mechanisms that is enhanced when adopting orthogonal instrumental platforms to expand metabolome coverage, while also reducing false discoveries by independent replication. Herein, we report the first inter-method comparison when using multisegment injec...
2023-08-22 | MTBLS3111 | MetaboLights
MetabolomicsUPLC-MS-Based Serum Metabolic Profiling Reveals Potential Biomarkers for Predicting Propofol Responsiveness in Females

Although previous studies have shown that certain factors interfere with the sensitivity of propofol, the mechanisms for interindividual variability in response to propofol remain unclear. This study aimed to screen the metabolites to predict patients' sensitivity to propofol and to identify meta...

2021-09-29 | MTBLS2311 | MetaboLights
ProteomicsIntegrating serum protein electrophoresis with mass spectrometry, a new workflow for M-protein detection and quantification
Serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE) are standard tools for multiple myeloma (MM) routine diagnostics. M-protein is a biomarker for MM that can be quantified with SPE and characterized with IFE. We have investigated combining SPE/IFE with targeted mass spectro...
ORGANISM(S): Homo sapiens (Human) 
2020-01-07 | PXD016325 | Pride
Serum protein electrophoresis Immunofixation electrophoresis Targeted mass spectrometry Multiple myeloma M-protein 
MetabolomicsIn Vitro 1H NMR Metabolic Profiles of Liver, Brain, and Serum in Rats After Chronic Consumption of Alcohol

The impact of alcohol on the body can be investigated with NMR spectroscopy in vitro, which can detect a wide range of metabolites but preparing samples includes tissue biopsy. Blood sampling is less invasive, but blood metabolic content might not reflect the changes occurring in other t...

2023-02-20 | MTBLS453 | MetaboLights
ProteomicsHigh-throughput plasma/serum proteomics by coupling nanoparticle protein corona-based sample preparation and capillary zone electrophoresis-tandem mass spectrometry
A high-throughput workflow for bottom-up proteomics (BUP) of human plasma using capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) and nanoparticle protein corona-assisted sample preparation is presented. The streamlined approach enabled the identification and quantification of hun...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-08-04 | PXD063043 | Pride
Nanoparticle protein corona Bottom-up proteomics High-throughput Cze-ms/ms Biomarker 
ProteomicsBAC-DROP: Rapid digestion of proteome fractionated via dissolvable polyacrylamide gel electrophoresis and its application to bottom-up proteomics workflow
The GeLC-MS workflow, which combines low-cost, easy-to-use SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with liquid chromatography-mass spectrometry (LC-MS), is very popular in current bottom-up proteomics. However, GeLC-MS requires that PAGE-separated proteins undergo overnight enzymatic diges...
ORGANISM(S): Homo Sapiens Hepatitis B Virus Bos Taurus 
2021-01-24 | PXD021489 | panorama
ProteomicsOrbitrap mass spectrometry and high-field asymmetric waveform ion mobility spectrometry (FAIMS) enable the indepth analysis of human serum proteoforms
In this study, we describe a straightforward protocol for intact proteoform sample preparation based on depletion of albumin and immunoglobulins followed by simplified fractionation of remaining serum proteins via polyacrylamide gel electrophoresis. After molecular weight-based fractionation, we sup...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-02 | MSV000092574 | MassIVE
ProteomicsSerum proteomics of multiple menstrual symptoms in female athlete
Menstrual symptoms, including primary dysmenorrhea and premenstrual syndrome, are significant problems affecting women’s health condition. However, their biological functions are still unclear. The objective of this study was to elucidate proteins related to menstrual symptoms with high-performance ...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD065313 | Pride
Human Serum Lc-ms Two-dimentional gel electrophoresis 
ProteomicsSerum proteomic pattern in female stress urinary incontinence
The pathophysiology of Stress Urinary Incontinence (SUI) is poorly understood. The aim of this study was to identify the serum proteomic profile in patients with SUI and to replicate findings from a preceding study in which a significant difference in the urinary proteome was identified. Serum samp...
ORGANISM(S): Homo sapiens (Human) 
2018-01-24 | PXD008553 | Pride
Urinary incotinence Proteomics Female Serum Stress 
MetabolomicsCE-MS Metabolomic and LC-MS Proteomic Analyses of Breast Cancer Exosomes Reveal Alterations in Purine and Carnitine Metabolism

A nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased ...

2024-11-14 | MTBLS11190 | MetaboLights
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