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ProteomicsSystematic identification of 20S proteasome targets
For many years, the ubiquitin-26S proteasome degradation pathway was considered the principal route for proteasomal degradation. However, it is now becoming clear that proteins can also be targeted for degradation by an ubiquitin-independent mechanism mediated by the core 20S proteasome itself. The ...
ORGANISM(S): Homo sapiens (Human) 
2023-12-21 | PXD044399 | Pride
Disorder Degradation 20s proteasome 
ProteomicsTracking Proteasome Degradation: A Cross-Organ Analysis via Intact degradomics Top-Down Mass Spectrometry
Identifying the peptides generated by the proteasome is essential for understanding its role in regulating critical cellular processes such as apoptosis, signaling, and immune responses. In this study, we introduce an enzymatic top-down mass spectrometry approach that allows real-time monitoring of ...
ORGANISM(S): Mus musculus (Mouse) 
2025-08-25 | PXD058121 | Pride
Mass spectrometry Top-down ms Proteasome Protein degradation 
ProteomicsDynamic regulation of the COP9 signalosome: Insights into the spatial localization and subunit versatility of the complex
HeLa cells were fracitonated into nuclear soluble, chromatin bound and cytosolic. Extracts were immunoprecipitated with a CSN3 antibody, then eluted with glycine. Proteins were digested, then enriched for phosphopeptides. Samples were analyzed on an LTQ-Orbitrap. Data was analyzed using the TRansOMi...
ORGANISM(S): Homo sapiens (Human) 
2019-11-25 | PXD000277 | Pride
Proteomics Human Phosphoproteomics Csn 
TranscriptomicsInferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters
Despite extensive research, our understanding of the rules according to which cis-regulatory sequences are converted into gene expression is limited We devised a method for obtaining parallel, highly accurate gene expression measurements from thousands of designed promoters and applied it to measure...
ORGANISM(S): Saccharomyces cerevisiae 
2012-05-20 | E-GEOD-37851 | biostudies-arrayexpress
TranscriptomicsUnraveling determinants of transcription factor binding outside the core binding site
Binding of transcription factors (TFs) to regulatory sequences is a pivotal step in the control of gene expression. Despite many advances in the characterization of sequence motifs recognized by TFs, our ability to quantitatively predict TF binding to different regulatory sequences is still limited....
ORGANISM(S): synthetic construct 
2015-03-04 | E-GEOD-66143 | biostudies-arrayexpress
TranscriptomicsExpression data from HeLa cells overexpressing AK125393
Expression of RAD51, a crucial player in homologous recombination (HR) and DNA double-strand break (DSB) repair, is dysregulated in human tumors, and can contribute to genomic instability and tumor progression. To further understand RAD51 regulation we functionally characterized a long non-coding (l...
ORGANISM(S): Homo sapiens 
2015-02-19 | E-GEOD-66062 | biostudies-arrayexpress
TranscriptomicsAlu sequences in undifferentiated human embryonic stem cells display high levels of A-to-I RNA editing
Adenosine to Inosine (A-to-I) RNA editing is a site-specific modification of RNA transcripts, catalyzed by members of the ADAR (Adenosine Deaminase Acting on RNA) protein family. RNA editing occurs in human RNA in thousands of different sites. Some of the sites are located in protein-coding regions ...
ORGANISM(S): Homo sapiens 
2010-07-29 | E-GEOD-19719 | biostudies-arrayexpress
UnknownGlobal hospital-based disease management of acute diverticulitis: a prospective, international cohort study.
Not available
S-EPMC12512976 | biostudies-literature
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