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ProteomicsEnrichment and quantitation of newly secreted proteins under serum condition by combining BONCAT and pulsed SILAC
Despite the increasing interest of secretome involved in paracrine/autocrine mechanisms, the majority of the mass spectrometric cell secretome studies have been performed using serum-free medium (SFM), due to low-abundance of secreted proteins and high-abundance of contaminating fetal bovine serum (...
ORGANISM(S): Homo sapiens (Human) 
2019-03-13 | PXD007529 | Pride
Boncat Serum Fbs Secretome Pulsed silac Lc-ms/ms 
OtherDiscovery of melanotransferrin as a serological marker of colorectal cancer by secretome analysis and quantitative proteomics.
Data from PeptideAtlas, [[http://www.peptideatlas.org/PASS/PASS00565 PASS00565]]. File: 100521_HCT-116_OG11.mzml. Published as part of . From the Abstract: {{i}} In order to discover serological colorectal cancer (CRC) markers, we analyzed cell line secretome to gather proteins of higher potential ...
ORGANISM(S): Homo_sapiens_viruses, Cow,human 
GPM11210029831 | GPMDB
ProteomicsShift of EMT gradient in 3D spheroid MSCs for activation of mesenchymal niche function
This project is described about the detailed characterization of 3D spheroid mesenchymal stem cells (MSCs). Specifically, authors showed the dynamic action towards EMT in 3D MSCs and the potential of EMT-enhanced “naïve” mesenchymal phenotype.
ORGANISM(S): Homo sapiens (Human) 
2018-06-25 | PXD006653 | Pride
Human Cell 3d-cultured Mesenchymal stem cell Lc-ms/ms 
ProteomicsSelective Enrichment of Newly Synthesized Proteins using Phos-tag Click Tip Enables Nascent Proteome Analysis in Influenza A Virus Infection
Bioorthogonal non-canonical amino acid tagging (BONCAT)-based approaches enable selective labeling of newly synthesized proteins (NSPs). We developed a workflow termed the “Phos-tag Click Tip” by integrating a phosphorylated variant of bicyclononyne (pBCN) with Phos-tag affinity purification to sele...
ORGANISM(S): Homo Sapiens (human) 
2026-09-09 | PXD059239 | JPOST Repository
ProteomicsDevelopment of parallel reaction monitoring mass spectrometry assay for the detection of human norovirus major capsid protein
Human Norwalk virus (HuNoVs), the most common etiological agents of acute gastroenteritis, are genetically diverse RNA viruses that frequently cause mass food poisoning internationally. In this study, we developed a parallel reaction monitoring (PRM) assay to simultaneously detect and identify repre...
ORGANISM(S): Homo Sapiens (human) Norwalk Virus 
2023-02-21 | PXD033981 | JPOST Repository
ProteomicsIdentification of serum prognostic biomarkers of severe COVID-19 using a quantitative proteomic approach
The COVID-19 pandemic is an unprecedented threat to humanity that has provoked global health concerns. Since the etiopathogenesis of this illness is not fully characterized, the prognostic factors enabling treatment decisions have not been well documented. Accurately predicting the progression of th...
ORGANISM(S): Homo Sapiens (human) 
2021-11-02 | PXD021702 | JPOST Repository
ProteomicsHuman serum spectral library for DIA mass spectrometry
For comprehensive human serum proteome analysis, we attempted to construct a serum spectral library for DIA-MS. Consequently, the customized spectral DIA library contained information on 23,196 peptides derived from 1,534 human serum proteins. We expect that this customized spectral DIA library will...
ORGANISM(S): Homo Sapiens (human) 
2021-11-10 | PXD027635 | JPOST Repository
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