Sort   by:  
 Page size 
ProteomicsComparing SILAC- and stable isotope dimethyl-labeling approaches for quantitative proteomics.
Comparison of SILAC and reductive dimethyl labeling; also applied both methods to affinity enrichment experiments with and without fractionation.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-29 | MSV000082868 | MassIVE
ProteomicsImproved SILAC Method for Double Labeling of Bacterial Proteome
Stable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with the advantages of reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quanti...
ORGANISM(S): Escherichia coli Staphylococcus aureus 
2022-02-28 | PXD011244 | Pride
Proteomics Double labeling Bacteria Silac 
ProteomicsImproved SILAC Method for Double Labeling of Bacterial Proteome
Stable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with the advantages of reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quanti...
ORGANISM(S): Staphylococcus aureus 
2022-03-02 | PXD011221 | Pride
Proteomics Double labeling Bacteria Silac 
ProteomicsQuantification of mitochondrial translation by pulse SILAC
We developed a simple method that combined biochemical mitochondria isolation and pulse SILAC approach to monitor the mitochondrial translation. Our approach allows us to quantify 12 out of the 13 mitochondrial translation products, the highest coverage among analogous methods reported, and provide ...
ORGANISM(S): Homo Sapiens (human) 
2022-12-28 | PXD022476 | JPOST Repository
ProteomicsTime-resolved analysis of proteome dynamics by TMT-SILAC hyperplexing
In this project, a more efficient methodology for obtaining multi-timepoint kinetic data for proteome-wide analyses of protein turnover is developed and used to globally measure the kinetics of protein turnover in primary human fibroblasts (HCA2-hTert). This approach takes advantage of the multiplex...
ORGANISM(S): Homo sapiens (Human) 
2016-12-14 | PXD004725 | Pride
Tmt-silac Protein degrdation Tmt Proteome dynamics Silac Human primary fibroblasts Protein synthesis Hyperplexing 
ProteomicsImproving SILAC quantification with data independent acquisition to investigate bortezomib-induced protein degradation
Stable isotope labeling by amino acids in cell culture (SILAC) coupled to data-dependent acquisition (DDA) is a common approach to quantitative proteomics with the desirable benefit of reducing batch effects during sample processing and data acquisition. More recently, using data-independent acquisi...
ORGANISM(S): Homo Sapiens 
2022-03-29 | PXD022659 | panorama
ProteomicsComprehensive characterization of protein turnover by comparative SILAC labeling analysis in 3T3-L1
A balance between the synthesis and degradation of proteins is referred to as protein turnover, which is crucial for cellular protein homeostasis. Proteome-wide analysis of protein turnover in adipocytes, which are well-known for storing energy and also being associated with obesity and metabolic di...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-18 | PXD037368 | Pride
Mass spectrometry Protein turnover Adipocytes Proteomics Silac 
TranscriptomicsAnalysis of HeLa cells after transfection with miR-1, miR-124 and miR-181.
Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS. Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124,...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-11968 | biostudies-arrayexpress
ProteomicsMaximally-multiplexed proteome quantification platform for isotopic metabolic and chemical labeling
Quantitative proteomic platforms based on precursor intensity in mass spectrometry (MS1-level) uniquely support in vivo metabolic labeling with superior quantification accuracy but suffers from limited multiplexity (≤3-plex) and frequent missing quantities. Herein, we present a maximally-multiplexed...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD009952 | Pride
Diethylation Heat shock Silac-6plex Protein quantification Hela cell Quantification Silac De-6plex Heat shock response 
UnknownEffective correction of experimental errors in quantitative proteomics using stable isotope labeling by amino acids in cell culture (SILAC).
Not available
S-EPMC3394155 | biostudies-literature
Sort   by:  
 Page size