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ProteomicsStage-specific protein regulation during somatic embryo development of Carica papaya L. ‘Golden’
Somatic embryogenesis is an important biotechnological technique for large-scale propagation of elite genotypes. Correlations of stage-specific compounds associated with somatic embryo development can help elucidate the ontogenesis of Carica papaya L. somatic embryos and improve tissue culture proto...
ORGANISM(S): Carica papaya 
2020-11-12 | PXD021107 | Pride
C. papaya Polyamines Somatic embryogenesis Label-free proteomics 
ProteomicsAlternative oxidase (AOX) seems to play a key role in embryogenic competence acquisition in Carica papaya L. calli
Understanding the mechanisms that endow a somatic cell with the ability to differentiate into a somatic embryo, which could result in numerous biotechnological applications, is still a challenge. The objective of this work was to identify some of the molecular and physiological mechanisms responsibl...
ORGANISM(S): Carica papaya 
2022-06-28 | PXD026311 | Pride
Somatic embryos Label-free proteomics Hormones Mitochondrial proteomics 
ProteomicsTime-dependent proteomic signatures associated with embry-ogenic callus induction in Carica papaya L.
The cost of Carica papaya production through seed-based propagation is increased by sex segregation, making in vitro techniques a more appealing option for clonal propagation. Inducing embryogenic callus with 2,4-dichlorophenoxyacetic acid (2,4-D) hold the potential to large-scale cloning, although ...
ORGANISM(S): Carica papaya 
2024-01-26 | PXD046521 | Pride
Bottom-up proteomics  carica papaya  embryogenic callus  somatic embryos  time-series analysis. 
ProteomicsDifferentially abundant proteins associated with heterosis in the primary roots of popcorn
Although heterosis has significantly contributed to increases in worldwide crop production, the molecular mechanisms regulating this phenomenon are still unknown. In the present study, we used a comparative proteomic approach to explore hybrid vigor via the proteome of both the popcorn L54 ♀ and P8 ...
ORGANISM(S): Zea mays (Maize) 
2018-05-17 | PXD009436 | Pride
Nano-lc-esi-ms/ms Progenesis Synapt g2 si Heterosis Popcorn Non-additive effect Shotgun proteomics 
ProteomicsLED lamps induce a differential regulation of proteins and enhance somatic embryo maturation in Carica papaya
The use of light-emitting diode (LED) lamps could be an alternative method to improve somatic embryogenesis, yielding more efficient somatic embryo development and maturation than the use of conventional fluorescent lamps. The aim of this work was to study the influence of light quality on the somat...
ORGANISM(S): Carica papaya 
2019-09-11 | PXD009785 | Pride
Clonal propagation Label-free proteomics Carica papaya l. Comparative proteomics led Somatic embryogenesis Light-emitting diode 
ProteomicsEmbryogenic competence acquisition in sugarcane callus is associated with differential H+ pump abundance and activity
Somatic embryogenesis is an important biological process in several plant species, including sugarcane. Proteomics approaches have shown that H+ pumps are differentially regulated during somatic embryogenesis; however, the relationship between H+ flux and embryogenic competence is still unclear. Thi...
ORGANISM(S): Saccharum officinarum 
2018-06-26 | PXD009858 | Pride
Somatic embryogenesis  ion-selective vibrating probe  saccharum ssp.  h+ pumps  microsomal proteomics 
ProteomicsPhosphorylation mediates hormonal, epigenetic and stress responses related to sugarcane somatic embryogenesis competence
In somatic embryogenesis, embryos are formed from a single or small group of somatic cells in response to stress and hormonal stimuli. In this study, a high-throughput phosphoproteomic analysis was used to identify and quantify phosphoproteins related to the acquisition of embryogenic competence in ...
ORGANISM(S): Saccharum hybrid cultivar SP80-3280 
2020-10-02 | PXD018054 | Pride
Abscisic acid Snrk1 Phosphoproteomics Histone deacetylase Totipotency 
ProteomicsLight spectra affect in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by changing the differential accumulation of proteins and polyamine contents
Light spectrum quality is an important signal for plant growth and development. We aimed to analyze the effects of different light spectra on in vitro shoot development and proteomic and polyamine (PA) profiles in shoots of Cedrela fissilis. Cotyledonary and apical nodal segments were grown under di...
ORGANISM(S): Cedrela fissilis 
2020-09-04 | PXD018020 | Pride
In vitro morphogenesis Fasta databank generation Polyamines Nodal segments Light-emitting diode lamp Proteomics Rna-seq 
ProteomicsSalt stress induces changes in the proteomic profile of micropropagated sugarcane shoots
Salt stress is one of the most common stresses in agricultural regions worldwide. In particular, sugarcane is affected by these conditions and there are still no cultivars that show high productivity allied with a tolerance to salt stress. Proteomic analysis is an important tool for elucidating impo...
ORGANISM(S): Saccharum sp. 
2017-04-26 | PXD006075 | Pride
Saccharum spp.  sugarcane  comparative proteomics Nacl-induced stress  abiotic stress. 
ProteomicsIntegrative proteomics and phosphoproteomics reveals phosphorylation networks involved in maintenance and expression of embryogenic competence in sugarcane callus
Sugarcane (Saccharum spp.) is one of the most important crops for sugar, biofuels and bioenergy production, becoming an important commodity in the world agricultural market for more than 100 countries. In this study, label-free quantitative proteomics and phosphoproteomics analyses were performed to...
ORGANISM(S): Saccharum hybrid cultivar SP80-3280 
2022-05-07 | PXD027388 | Pride
Label-free phosphoproteomics Embryo development Somatic embryogenesis Sugarcane 
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