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ProteomicsHigh-throughput Proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers
Skeletal muscles are composed of a heterogeneous collection of fiber types with different physiological adaption in response to a stimulus and disease-related conditions. Each fiber has a specific molecular expression of myosin heavy chain molecules (MyHC). So far MyHCs are currently the best marker...
ORGANISM(S): Mus musculus (Mouse) 
2021-02-02 | PXD017755 | Pride
Muscle fiber proteomics Protein turnover Phosphoproteomics Myhc profiling 
ProteomicsSingle muscle fiber proteomics reveals unexpected mitochondrial specialization
Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here we developed a high sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and u...
ORGANISM(S): Mus musculus (Mouse) 
2015-02-05 | PXD001641 | Pride
Metabolism Muscle fibers Single cell Exercise Mitochondria 
MetabolomicsSerine synthesis pathway enzyme PHGDH is critical for muscle cell biomass, anabolic metabolism and mTORC1 signaling

Cells use glycolytic intermediates for anabolism e.g., via the serine synthesis and pentose phosphate pathways. However, we still understand poorly how these metabolic pathways contribute to skeletal muscle cell biomass generation. The first aim of this study was therefore to identify enzymes tha...

2025-03-21 | MTBLS8854 | MetaboLights
MetabolomicsBacteroides acidifaciens Derived Succinate Mediates Gynostemma pentaphyllum Polysaccharides' Effects on Muscle Fiber Switching and Exercise Endurance

Enhancing exercise endurance holds significant practical implications for boosting the physical fitness of the general population to address daily challenges and improving the physical capabilities and quality of life of patients with relevant diseases. Polysaccharides from Gynostemma pentaphyllu...

2025-10-28 | MTBLS13229 | MetaboLights
TranscriptomicsSingle-nucleus transcriptomics reveals functional compartmentalization in syncytial skeletal muscle cells
Syncytial skeletal muscle cells contain hundreds of nuclei in a shared cytoplasm. Using single nucleus RNA-sequencing (snRNAseq) of isolated nuclei from muscle fibers, we investigated nuclear heterogeneity and transcriptional dynamics in uninjured and regenerating muscle.
ORGANISM(S): Mus musculus 
2020-10-07 | E-MTAB-8623 | biostudies-arrayexpress
ProteomicsSingle muscle fiber proteomics reveals remodeling of focal adhesions in response to muscle unloading and microgravity
Spaceflight causes loss of muscle mass and strength, metabolic remodeling and insulin resistance, contributing to severe challenges for astronaut health. We used highly sensitive proteomics to detail single fiber type-specific molecular remodeling caused by muscle unloading in subjects undergoing pr...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD028435 | Pride
Proteomics Microgravity Muscle 
ProteomicsSingle muscle fiber proteomics reveals fiber type-specific features of human muscle aging
Skeletal muscle is a key tissue in human aging, which affects different muscle fiber types unequally. We developed a highly sensitive single muscle fiber proteomics workflow to study human aging and show that the senescence of slow and fast muscle fibers is characterized by diverging metabolic and p...
ORGANISM(S): Homo sapiens (Human) 
2017-06-14 | PXD006182 | Pride
Muscle fibers Skeletal muscle Aging 
TranscriptomicsTranscription profiling of skeletal muscle from wild type and PGC-1 beta transgenic mice
Skeletal muscle must perform a wide range of kinds of work, and different fiber types have evolved to accommodate these different tasks. The attributes of fibers are determined in large part by the coordinated regulation of oxidative capacity, as reflected by mitochondrial content, and the specific...
ORGANISM(S): Mus musculus 
2007-04-02 | E-MEXP-939 | biostudies-arrayexpress
GenomicsExpression data from wild-type single muscle fibers
Expression data from wild-type single muscle fibers
PRJNA557650 | ENA
ProteomicsMuscle proteomics of mitochondrial disorders
Mitochondrial DNA mutations progressively compromise the respiratory chain of skeletal muscle, resulting in a mosaic of metabolically healthy and defective fibers. This heterogeneity is the best diagnostic signpost of mitochondrial disorders. However, the single fiber investigation of its molecular...
ORGANISM(S): Homo sapiens (Human) 
2019-11-15 | PXD010489 | Pride
Laser microdissection Single muscle fibers Q exactive hf Human muscle Mitochondrial disorders 
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