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ProteomicsAnalysis of the substrate specificities and reaction kinetics of the yeast oligosaccharyltransferase isoforms
Oligosaccharyltransferase (OST) catalyzes the central step in N-linked protein glycosylation, the transfer of a pre-assembled oligosaccharide from its lipid carrier onto asparagine residues of secretory proteins. The prototypic hetero-octameric OST complex from the yeast Saccharomyces cerevisiae exi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-05-19 | PXD024590 | Pride
Site-occupancy Yeast oligosaccharyltransferase Substract specificity 
ProteomicsN-glycosylation site occupancy in human prostaglandin H synthases expressed in Pichia pastoris
Prostaglandin H synthases (PGHSs) are N-glycosylated membrane proteins that catalyse the committed step in prostaglandin synthesis. Unlike PGHS-2, the production of recombinant PGHS-1 in non-mammalian expression systems is complicated. The majority of the heterologous enzyme is inactive due to misfo...
ORGANISM(S): Homo sapiens (Human) Komagataella pastoris (Yeast) (Pichia pastoris) 
2014-08-18 | PXD000965 | Pride
Proteomics Recombinant protein Pghs N-glycosylation Lc-ms/ms 
ProteomicsGlobal mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line
Mucin-type-O-glycosylation on proteins is integrally involved in human health and disease and is coordinated by an enzyme family of 20 N-acetylgalactosaminyltransferases (GalNAc-Ts). Detailed knowledge on the biological effects of site-specific O-glycosylation is limited due to lack of information o...
ORGANISM(S): Homo sapiens (Human) 
2022-10-22 | PXD036791 | Pride
Galnt   fibronectin  ldlr  lrp  col17a1 Differential glycoproteomics. Crispr 3d tissue models  oragnoid 
ProteomicsGlobal, site-resolved analysis of ubiquitylation occupancy and turnover rate reveals systems properties
Ubiquitylation regulates most proteins and biological processes in a eukaryotic cell. However, the site-specific occupancy (stoichiometry) and turnover rate of ubiquitylation have not been quantified. Here we present an integrated picture of the global ubiquitylation site occupancy and half-life. Ub...
ORGANISM(S): Homo sapiens (Human) 
2024-04-15 | PXD037009 | Pride
Human Ubiquitylation Dynamics Ptms Stoichiometry Half-life 
UnknownSite occupancy calibration of taxane pharmacology in live cells and tissues.
Not available
S-EPMC6275538 | biostudies-literature
UnknownLarge-scale quantitative glycoproteomics analysis of site-specific glycosylation occupancy.
Not available
S-EPMC3463725 | biostudies-literature
UnknownA sensitive green fluorescent protein biomarker of N-glycosylation site occupancy.
Not available
S-EPMC3448770 | biostudies-literature
UnknownGlobal, site-resolved analysis of ubiquitylation occupancy and turnover rate reveals systems properties.
Not available
S-EPMC11136510 | biostudies-literature
GenomicsRibosome stalling, spacing and A-site occupancy impact translation and co-translational mRNA decay in plants
Ribosome stalling, spacing and A-site occupancy impact translation and co-translational mRNA decay in plants
PRJNA1451156 | ENA
UnknownSite occupancy by American martens and fishers in temperate deciduous forests of Quebec.
Not available
S-EPMC9936503 | biostudies-literature
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