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ProteomicsHuman connective tissue mast cell proteome
Mast cells are hematopoietic cells that reside preferentially in tissues exposed to internal and external environments. Mast cells sense immunological, inflammatory and environmental stimuli, and can be activated to release granules and generate inflammatory mediators. Mast cell-derived products con...
ORGANISM(S): Homo sapiens (Human) 
2020-02-12 | PXD014978 | Pride
Human Fat mast cell Skin mast cell 
TranscriptomicsscRNAseq of freshly dissociated and cultured skin biopsies
We describe new optimised skin tissue dissociation protocol to digest ex vivo cultured and fresh human skin samples. Using scRNAseq analysis we created skin atlas composition of fresh and cultured skin samples and integrate it with other publicly available skin tissue scRNAdata to compare the qualit...
ORGANISM(S): Homo sapiens 
2022-08-04 | E-MTAB-11509 | biostudies-arrayexpress
TranscriptomicsHuman dendritic cell subsets in acute skin inflammation
An unexpected BDCA-2+CD123+CD1a+ dendritic cell (DC) subset comprised a major DC population in acute human sterile skin inflammation. Single-cell RNAseq showed these to be activated DC able to express markers normally associated with plasmacytoid DC, prompting a re-evaluation of previous studies.
ORGANISM(S): Homo sapiens 
2019-12-01 | E-MTAB-8498 | biostudies-arrayexpress
ProteomicsGlobal phosphoproteomics in SCF stimulated human skin mast cells
Global phosphoproteomics can reveal signaling cascades in unprecedented detail. The SCF/KIT axis represents a vital hematopoietic receptor tyrosine kinase (RTK) system with gain-of-function mutations prompting the development of mastocytosis and leukemia. Here, we employed a label-free phosphoproteo...
ORGANISM(S): Homo sapiens (Human) 
2022-03-31 | PXD028245 | Pride
Phosphoproteome Human Scf Mast cells 
ProteomicsGlobal phosphoproteomics elicited by the SCF/KIT axis in human skin mast cells unveil capicua as a potent KIT repressor
Global phosphoproteomics can reveal signaling cascades in unprecedented detail. The SCF/KIT axis represents a vital hematopoietic receptor tyrosine kinase (RTK) system with gain-of-function mutations prompting the development of mastocytosis and leukemia. Here, we employed a label-free phosphoproteo...
ORGANISM(S): Homo sapiens (Human) 
2022-11-15 | PXD028252 | Pride
Phosphoproteome Human Scf Mast cells 
Transcriptomicssteady-state mast cell signatures in mouse PEC and skin
Expression of selected genes in PEC or skin from wild type or mast cell-deficient mice. Specific genes were selected from the whole array by gene name and their expression levels were compared. All other genes have been excluded from the data files.
ORGANISM(S): Mus musculus 
2011-11-17 | E-MTAB-838 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human grafted engineered skin substitutes compared with intact human
The goal of the experiment: To characterize the dynamic gene expression profile of engineered human skin in vitro and after grafting, and compare with expression profile of uninjured human skin. Bioengineered skin substitutes can facilitate wound closure in massively burned patients, but deficiencie...
ORGANISM(S): Homo sapiens 
2009-09-07 | E-GEOD-17539 | biostudies-arrayexpress
ProteomicsBone marrow-derived mast cells +- IL-33 48h
Mast cells are tissue resident granulocytes which are most abundant at the interface between tissues and the external environment, such as around blood vessels, in the skin or mucosal surfaces in the lungs and gut. Pathologically they are involved in allergic reactions and anaphylaxis, however they ...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-11 | PXD033759 | Pride
Granulocyte Mouse Lc-ms Mast cell Il-33 
TranscriptomicsMicroarray of reconstructed epidermis from skin models made with cells from African and Caucasian Skin type
The aim of this study was to analyze the in vitro biological epidermal processes occurring in reconstructed skins using cells from breast skin of African and Caucasian skin type color. A exploration of mRNA expression levels in the epidermis of reconstructed skin was undertaken to elucidate the diff...
ORGANISM(S): Homo sapiens 
2020-08-10 | E-MTAB-9433 | biostudies-arrayexpress
TranscriptomicsMultiscale spatial mapping of cell populations across anatomical sites in healthy human skin and basal cell carcinoma - Spatial Transcriptomics - 10X Visium
Our understanding of how human skin cells differ according to anatomical site and tumour formation is limited. To address this we have created a multi-scale spatial atlas of healthy skin and basal cell carcinoma (BCC), incorporating in vivo optical coherence tomography, single cell RNA sequencing, s...
ORGANISM(S): Homo sapiens 
2023-12-22 | E-MTAB-13085 | biostudies-arrayexpress
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