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MetabolomicsMetabolomics of SLE-associated podocytes
To characterize metabolic changes in SLE-associated podocytes, 1 × 10^6 podocytes were stimulated for 24 hours with immune complexes (ICs) isolated from patients with systemic lupus erythematosus (SLE) or with IgG from healthy donors as a control. The cells were then collected and analyzed by untarg...
2026-09-23 | MTBLS15778 | MetaboLights
TranscriptomicsDifferentially expressed transcripts in SLE blood monocytes
To better characterize the molecules that could potentially confer antigen presenting capacity to SLE monocytes, we assessed their gene expression profile. Blood monocytes from five healthy controls and five pediatric SLE patients were isolated using CD14+ selection. Because drugs used to treat SLE ...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-46907 | biostudies-arrayexpress
TranscriptomicsTranscripts induced by exposure to SLE serum in healthy blood monocytes
Monocytes from 3 healthy donors were cultured for 6 hours in the presence of 20% serum from three newly diagnosed, untreated SLE patients. Microarray analysis was then performed upon normalizing the gene expression levels of samples incubated with SLE sera to those incubated with autologous serum. M...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-46920 | biostudies-arrayexpress
TranscriptomicsThe multifaceted balance of TNF-a and type I / II interferon responses in SLE and RA: how monocytes manage the impact of cytokines
Many cytokines are involved in the pathogenesis of autoimmune diseases and are recognized as relevant therapeutic targets to attenuate inflammation, such as TNFα in RA and IFNα/γ in SLE. To relate the transcriptional imprinting of cytokines in a cell type-specific and disease-specific manner, we gen...
ORGANISM(S): Homo sapiens 
2012-06-13 | E-GEOD-38351 | biostudies-arrayexpress
TranscriptomicsDifferentially expressed transcripts in SLE blood monocytes according to their capacity to induce mixed lymphocytic reaction
We screened SLE monocytes from 19 SLE patients and selected 4 that induced CD4+ T cell proliferation in vitro and 4 that did not. CFSE labeled CD4-T cells (105) were incubated with SLE monocytes (2 x 104). Cells were harvested at 6 hours for RNA extraction. We screened SLE monocytes from 19 SLE pati...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-46917 | biostudies-arrayexpress
TranscriptomicsDivergent genome wide transcriptional profiles from immune cell subsets isolated from SLE patients with different ancestral backgrounds
Background/Purpose: Systemic lupus erythematosus (SLE) is a complex multi-system autoimmune disease of uncertain etiology. Patients from different ancestral backgrounds demonstrate differences in clinical manifestations and autoantibody profiles. In this study we examined genome-wide transcription...
ORGANISM(S): Homo sapiens 
2015-05-15 | E-GEOD-55447 | biostudies-arrayexpress
GenomicsGenome-wide profiling of target genes for the systemic lupus erythematosus-associated transcription factors IRF5 and STAT4
IRF5 and STAT4 are strongly associated with human systemic lupus erythematosus (SLE). By performing chromatin immunoprecipitation-sequencing (ChIP-Seq) in human peripheral blood mononuclear cells (PBMCs), we identified more than 7000 target genes for IRF5 and STAT4 in stimulated PBMCs. Contrarily, w...
ORGANISM(S): Homo sapiens 
2012-06-08 | E-GEOD-38567 | biostudies-arrayexpress
TranscriptomicsSLE lupus RNA-seq
RNA-seq of systemic lupus erythematosus (SLE) whole blood and healthy controls to determine the gene expression changes in these patients. RNA-seq of PAXgene blood from SLE and healthy donors.
ORGANISM(S): Homo sapiens 
2015-09-16 | E-GEOD-72509 | biostudies-arrayexpress
ProteomicsEnrichment of complement, immunoglobulins, and autoantibody targets in the proteome of platelets from patients with Systemic Lupus Erythematosus (SLE).
SLE is a complex disease characterized by autoimmunity towards apoptotic cells, excessive amounts of circulating immune complexes and complement activation. Decreased platelet size has been observed in SLE and their non-hemostatic functions may play an active role in the disease. Our main objective ...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD031202 | Pride
Lc-msms Sle Thrombocytes 
TranscriptomicsTranscription profiling of human separated leukocyte subsets in SLE and vasculitis
Objective: To optimize a strategy for identifying gene expression signatures differentiating SLE and anti-neutrophil cytoplasmic antibody-associated vasculitis that provide insight into the pathogenesis and identify biomarkers.
Methods: Forty four vasculitis patients, 13 SLE patients and 25 age...
ORGANISM(S): Homo sapiens 
2010-06-02 | E-MTAB-145 | biostudies-arrayexpress
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