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MetabolomicsSmall molecule produced by Photorhabdus interferes with ubiquinone biosynthesis in Gram-negative bacteria

We report identification of 3,6-dihydroxy-1,2-benzisoxazole (DHB) in a screen of Photorhabdus and Xenorhabdus, whose symbiotic relationship with eukaryotic nematodes favors secondary metabolites that meet several requirements matching those for clinically useful antibiotics. DHB is produced by Ph...

2024-09-06 | MTBLS10720 | MetaboLights
MetabolomicsSearching for Drug Synergy Against Cancer Through Polyamine Metabolism Impairment: Insight Into the Metabolic Effect of Indomethacin on Lung Cancer Cells
Non-small cell lung cancer (NSCLC) is the most lethal and prevalent type of lung cancer. In almost all types of cancer, the levels of polyamines (putrescine, spermidine, and spermine) are increased, playing a pivotal role in tumor proliferation. Indomethacin, a non-steroidal anti-inflammatory drug, ...
2021-02-08 | MTBLS873 | MetaboLights
ProteomicsMembrane-Based Affinity Purification to Identify Target Proteins of a Small-Molecule Drug
We employ MaxLFQ to relatively quantify fold changes of proteins in two groups.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-05-27 | MSV000085483 | MassIVE
ProteomicsAnalysis of Immune Checkpoint Drug Targets and Tumor Proteotypes in Non-Small Cell Lung Cancer
New therapeutics targeting immune checkpoint proteins have significantly advanced treatment of non-small cell lung cancer (NSCLC), but protein level quantitation of drug targets presents a critical problem. We used multiplexed, targeted mass spectrometry (MS) to quantify the immunotherapy target pr...
ORGANISM(S): Homo Sapiens 
2020-06-22 | PXD019061 | panorama
Genomics"Drug-seq": An Approach to Identify the Genomic Targets of Chemicals Identified by Functional Phenotypic Screens [Drug-seq]
Here we report an approach that has permitted us to uncover the sites and mechanisms of action of a drug, referred to as SD70, initially identified by phenotypic screening for inhibitors of ligand and genotoxic stress-induced translocations in prostate cancer cells. Based on synthesis of a derivatiz...
ORGANISM(S): Homo sapiens 
2014-07-01 | E-GEOD-55901 | biostudies-arrayexpress
Proteomicsidentification of the protein targets of a small molecule
Mass spectrometry-based chemical proteomic approaches using limited proteolysis for analysis of the interactions between a small molecule and its protein target(s). DARTS (Drug Affinity Responsive Target Stability) has been used in this study on Hela cell lysate. Ubiquitin carboxyl-terminal hydrolas...
ORGANISM(S): Homo sapiens (Human) 
2024-01-15 | PXD048098 | Pride
Hela cells Orbitrap Lysate Lc-ms/ms 
MetabolomicsApoptosis-inducing effects of aqueous extract of Eleutherococcus senticosus on non-small cell lung cancer cell proliferation

Non-small cell lung cancer is a malignant tumor with high morbidity and mortality worldwide. Eleutherococcus senticosus can induce apoptosis in non-small cell lung cancer cells, but the mechanism remains unclear. This study aimed to elucidate the role of Eleutherococcus senticosus in inducing apo...

2025-05-12 | MTBLS10545 | MetaboLights
TranscriptomicsA genome-wide and dose-dependent inhibition map of androgen receptor binding by small molecules reveals its regulatory program upon antagonism
The androgen receptor plays a critical role throughout the progression of prostate cancer and is an important drug target for this disease. While chromatin immunoprecipitation coupled with massively parallel sequencing (ChIP-Seq) is becoming an essential tool in studying transcription and chromatin...
ORGANISM(S): Homo sapiens 
2013-12-31 | E-GEOD-32892 | biostudies-arrayexpress
MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
ProteomicsSelective ubiquitination of drug-like small molecules by the ubiquitin ligase HUWE1
The ubiquitin system regulates myriad pathways in eukaryotic physiology by modifying specific substrate proteins. The recognition of the substrates and the assembly of ubiquitin signals on them – key specificity determinants in ubiquitin signaling - are determined by the diversified class of ubiquit...
ORGANISM(S): Homo sapiens (Human) 
2025-09-03 | PXD063550 | Pride
Small-molecule inhibitor Hect e3 Mass spectrometry Enzymology Click chemistry Non-protein ubiquitination 
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