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ProteomicsSupercharging-Enhanced nDIA-MS Enables Global Profiling of Drug-Induced Proteome Solubility Shifts (solubility proteomics)
Mass spectrometry (MS)-based proteomics has become indispensable for high-throughput quantitation of protein expression. However, protein function is regulated by a variety of factors beyond abundance alone. Here, we optimized narrow-window, data-independent ac-quisition (nDIA) MS with 3% DMSO as a ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-12 | PXD064185 | Pride
Ml-792 Proteome Solubility shift Mg132 
ProteomicsIon-based Proteome Integrated Solubility Alteration Assay for System-wide Profiling of Protein-Molecule Interactions - full curve data
Unbiased drug target engagement deconvolution and mechanism of action elucidation are major challenges in drug development. Modification-free target engagement methods, such as thermal proteome profiling, have gained increasing popularity over the last several years. However, these methods have limi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD033081 | Pride
Proteomics Mechanism of action Drug target deconvolution Thermal protein profiling Cancer 
ProteomicsIon-based Proteome Integrated Solubility Alteration Assay for System-wide Profiling of Protein-Molecule Interactions
Unbiased drug target engagement deconvolution and mechanism of action elucidation are major challenges in drug development. Modification-free target engagement methods, such as thermal proteome profiling, have gained increasing popularity over the last several years. However, these methods have limi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD030695 | Pride
Proteomics Mechanism of action Drug target deconvolution Thermal protein profiling Cancer 
ProteomicsSolubility based mechanistic profiling of combinatorial drug therapy
Acute myeloid leukemia (AML) poses a major clinical challenge due to its genetic diversity, relapse rates, and chemotherapy toxicity. While rational drug combinations improve efficacy, their mechanisms remain poorly understood. We introduce CoPISA (Proteome Integral Solubility/Stability Alteration A...
ORGANISM(S): Homo sapiens (Human) 
2026-02-12 | PXD066812 | Pride
Combination therapy Acute myeloid leukaemia Proteome integral solubility alteration assay for combinations Copisa 
ProteomicsSupercharging-Enhanced nDIA-MS Enables Global Profiling of Drug-Induced Proteome Solubility Shifts (co-solvent)
Mass spectrometry (MS)-based proteomics has become indispensable for high-throughput quantitation of protein expression. However, protein function is regulated by a vari-ety of factors beyond abundance alone. Here, we optimized narrow-window, data-independent acquisition (nDIA) MS with 3% DMSO as a ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-11 | PXD064180 | Pride
Mnba Dmso Supercharging Ndia-ms 
ProteomicsSystem-wide profiling of drug residence time by proteome integral solubility alteration assay
Most drugs used in the clinic and drug candidates target multiple proteins, and thus detailed characterization of their efficacy targets is required. While current methods rely on quantitative measurements at thermodynamic equilibrium, kinetic parameters such as the residence time of a drug on its t...
ORGANISM(S): Homo sapiens (Human) 
2023-01-06 | PXD034351 | Pride
Target engagement Drug development Thermal proteome profiling. Efficacy target Lead compound optimization Target prioritization Binding affinity Precision medicine Target occupancy 
UnknownProteome-wide solubility and thermal stability profiling reveals distinct regulatory roles for ATP.
Not available
S-EPMC6411743 | biostudies-literature
GenomicsNot1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events
Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events
PRJNA798390 | ENA
UnknownIon-Based Proteome-Integrated Solubility Alteration Assays for Systemwide Profiling of Protein-Molecule Interactions.
Not available
S-EPMC9118197 | biostudies-literature
ProteomicsProteome-wide solubility and thermal stability profiling reveals distinct regulatory roles for ATP - SPP datasets
Nucleotide triphosphates (NTPs) regulate numerous biochemical processes in cells as (co-)substrates, allosteric modulators, biosynthetic precursors, and signaling molecules1-3. Apart from its roles as energy source fueling cellular biochemistry, adenosine triphosphate (ATP), the most abundant NTP in...
ORGANISM(S): Homo sapiens (Human) 
2019-03-11 | PXD012356 | Pride
Atp Jurkat Spp 
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