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A fast and sensitive Direct Extraction (DE) method developed in our group can efficiently extract proteins in 30 min from a 5 cm-long hair strand. Previously we coupled DE to downstream analysis using gel electrophoresis followed by in-gel digestion, which can be time-consuming. In searching for a b...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-10-26 | MSV000093203 | MassIVE
The optimal conditions and procedures for efficient and reproducible protein extraction of FFPE tissues have not yet been standardised and new sensitive techniques are continually being developed and improved upon. To our knowledge, there is no general agreement as to the choice of detergent or buff...
ORGANISM(S): Homo sapiens (Human) 
2022-02-15 | PXD014419 | Pride
The choice of growth media is a very important consideration of any cell-based proteomics experiment. Alterations thereof may result in differences in basal proteomes simply due to disparities in the metabolite composition of the media. We investigate the effect of growth media on the proteomes of t...
ORGANISM(S): Schizosaccharomyces pombe 927 Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2021-05-11 | PXD020816 | Pride
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of coIP samples.
ORGANISM(S): Homo Sapiens (human) 
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of 100ng of HEK293T proteins, coIP samples, and serum extracellular vesicles, and further challenged label-free single-cell proteomics o...
ORGANISM(S): Homo Sapiens (human) Bos Taurus 
Parkinson’s disease (PD) is increasingly recognized as a heterogeneous disorder, with rapid eye movement sleep behavior disorder (RBD) serving as a key prodromal marker for a distinct, aggressive subtype. This study investigates the plasma neuron-derived extracellular vesicle (EV) proteome to deline...
ORGANISM(S): Homo sapiens (Human) 
2026-08-27 | PXD076025 | Pride
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