Sort   by:  
 Page size 
ProteomicsKDM3A and KDM3B Maintain Naïve Pluripotency Through the Regulation of Alternative Splicing
Histone modifying enzymes play a central role in maintaining cell identity by establishing a conducive chromatin environment for lineage specific transcription factor activity. Pluripotent embryonic stem cells (ESCs) identity is characterized by lower abundance of gene repression associated histone ...
ORGANISM(S): Mus musculus (Mouse) 
2025-06-23 | PXD042735 | Pride
Flag-ip Mescs Histone demethylases 
ProteomicsCompartmentalization of HP1 proteins in pluripotency acquisition and maintenance
Embryonic stem cells (ESCs) have a hyperdynamic chromatin structure characterized by fewer discrete foci of heterochromatin protein 1 family of proteins (HP1) compared to somatic cells. During reprogramming of somatic cells, depletion of HP1γ early, reduced, while depletion later, enhanced the gener...
ORGANISM(S): Mus musculus (Mouse) 
2017-12-13 | PXD006817 | Pride
Pluripotency Ipsc Hp1 Esc 
TranscriptomicsRole of the reprogramming factors in inducing pluripotency
Induced pluripotent stem (iPS) cells can be obtained from fibroblasts by expression of Oct4, Sox2, Klf4, and c-Myc. To determine how these factors induce this change in cell identity, we carried out genomewide promoter analysis of their binding in iPS and partially reprogrammed cells. Most targets i...
ORGANISM(S): Mus musculus 
2010-05-06 | E-GEOD-14012 | biostudies-arrayexpress
TranscriptomicsExpression data from pre-iPSCs with a control, histone methyltransferase or Cbx3 (HP1g) knockdown
Transition from a partially reprogrammed pre-iPSC state to iPSC state can be achieved by modulating levels of histone modifying enzymes or proteins that can bind to histone modifications We used microarrays to determine the gene expression profile of pre-iPSCs depleted for either 3 histone methyltr...
ORGANISM(S): Mus musculus 
2013-06-05 | E-GEOD-44084 | biostudies-arrayexpress
TranscriptomicsHistone H3 lysine 56 acetylation is linked to the core transcriptional network in human embryonic stem cells
Lysine 56 acetylation in the helical core of histone H3 opens yeast chromatin and enables histone gene transcription, DNA replication, DNA repair, and prevents epigenetic silencing. While K56Ac is globally abundant in yeast and flies its presence has been uncertain in mammals. We show here using mas...
ORGANISM(S): Homo sapiens 
2009-02-09 | E-GEOD-14749 | biostudies-arrayexpress
TranscriptomicsExpression data from Chd1-deficient mouse ES cells (E14 cell lines) and genome-wide binding of Chd1 in parental ES cells
We used microarrays to study the effect of Chd1 loss of function in mouse ES cells. We used ChIP-chip to analyze genome-wide binding of Chd1 in normal ES cells. Mouse Embryonic Stem (ES) cells were infected with a lentiviral vector (pSicoR-mCherry) for expression of a shRNA against Chd1 and GFP (as ...
ORGANISM(S): Mus musculus 
2009-08-13 | E-GEOD-16462 | biostudies-arrayexpress
Sort   by:  
 Page size