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Deletion of the flap loop in the Rp2 subunit of human RNAPII did not alter the ability of human RNAPII to initiate transcription or elongate the initiated transcripts in vivo and in vitro, and was also dispensable for elongation factor-mediated enhancement and inhibition of transcript elongation in ...
ORGANISM(S): Homo sapiens 
IP-MS detection of proteins pulled down by Rpd3-FLAG by mass spectrometry
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-02-07 | PXD039051 | Pride
IP-MS detection of proteins pulled down by Ino80-FLAG by mass spectrometry
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-02-07 | PXD039052 | Pride
Proteins that may interact with HERD-1 were identified with Flag IP-mass in animals, which were tagged with 3xflag::GFP before the stat condon of HERD-1 in C.elegans.
ORGANISM(S): Caenorhabditis elegans 
2024-07-14 | PXD051885 | Pride
The goal of this project is to identify interacting proteins of Flag-tagged STING before and after cGAMP stimulation. STING knockout (STING-KO) BJ cells stably re-expressing Flag-STING were treated with 1 M cGAMP and whole cell lysates were harvested 30 min later for IP with anti-Flag M2 affinity ge...
ORGANISM(S): Homo sapiens (Human) 
2025-12-07 | PXD059426 | Pride
The ectomesenchyme generates much of the craniofacial skeleton, sutures, and diverse connective tissues in the mammalian head, yet its derivation from embryonic stem cells (ESCs) and the underlying molecular drivers remain poorly defined. Here, we identified Dlx2 as a key regulator that efficiently ...
ORGANISM(S): Mus musculus (Mouse) 
2025-11-06 | PXD064236 | Pride
We performed immunoprecipitation followed by LC-MS/MS (IP-MS) in mouse embryonic stem cells in which endogenous RLF or ZFP292 was tagged with FLAG. This approach enabled the identification of proteins associated with RLF/ZFP292. The dataset provides a resource to investigate the composition of RLF/Z...
ORGANISM(S): Mus musculus (Mouse) 
2026-03-21 | PXD075910 | Pride
To explore the potential targets of Aurora-A in glycolytic regulation
ORGANISM(S): Homo sapiens (Human) 
2019-11-21 | PXD016110 | Pride
Triplicate FLAG-Immunoprecipitations of FLAG-tagged FBXO7 constructs (full length (1-522), ∆N (169-522), ∆C (1-406), and ∆N∆C (169-406)) overexpressed in FBXO7-/- PSMF1-/- HEK293T cells. FBXO7∆N∆C co-overexpressed with full length PI31(1-271) is also included. Parental cells lacking tagged FBXO7 are...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD077028 | Pride
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