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TranscriptomicsThe Rpd3 Core Complex is a Chromatin Stabilization Module
The S. cerevisiae Rpd3 large (Rpd3L) and small (Rpd3S) histone deacetylase (HDAC) complexes are prototypes for understanding transcriptional repression in eukaryotes [1]. The current view is that they function by deacetylating chromatin, thereby limiting accessibility of transcriptional factors to t...
ORGANISM(S): Saccharomyces cerevisiae 
2012-01-01 | E-GEOD-33829 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type and rpd3- yeast cells in response to osmotic stress
Comparison of wild type and rpd3- S. cerevisiae cells in response to osmotic stress. Wild type (MATalpha ura3 leu2 his3) and rpd3- (MATalpha ura3 leu2 his3 rpd3::KANMX) were grown to OD660=1 in YPD media and then subjected or not to a brief osmotic stress (5 or 20 min / 0.4 M NaCl). Cells were colle...
ORGANISM(S): Saccharomyces cerevisiae 
2004-01-22 | E-MEXP-48 | biostudies-arrayexpress
ProteomicsCoRest’s acetylation acts as a temporal gate for activity-dependent transcription, limiting behavioral flexibility in Drosophila
In this project, to identify the proteins interacting with Rpd3 in the Drosophila mushroom body, the tandem affinity-purification(TAP) was performed. After in-solution digestion, the purified protein complex was analyzed by LC-MS/MS analysis using Orbitrap velos pro. PTM analysis was also performed...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2020-12-07 | PXD021294 | JPOST Repository
ProteomicsQuantitative analysis of proteome reveals the roles of Rpd3 and Sir2 in asexual development, virulence and multiple stress responses in Beauveria bassiana.
Lysine acetylation is one of the most prevalent post-translational modifications and has since been found to regulate histone functions and affect chromatin structure and gene expression. Here, we charactered Rpd3, a histone deacetylase of Beauveria bassiana, played important roles in the deacetylat...
ORGANISM(S): Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus) (Tritirachium shiotae) 
2021-08-12 | PXD006713 | Pride
Beauveria bassiana Rpd3 Lc-ms/ms. Proteome Sir2 
ProteomicsQuantitative analysis of acetylome reveals the roles of Rpd3 and Sir2 in asexual development, virulence and multiple stress responses in Beauveria bassiana.
Lysine acetylation is one of the most prevalent post-translational modifications and has since been found to regulate histone functions and affect chromatin structure and gene expression. Here, we charactered Rpd3, a histone deacetylase of Beauveria bassiana, played important roles in the deacetylat...
ORGANISM(S): Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus) (Tritirachium shiotae) 
2021-08-12 | PXD006714 | Pride
Acetylome Beauveria bassiana Rpd3 Lc-ms/ms. Sir2 
ProteomicsSaccharomyces cerevisiae，IP-MS detection of proteins pulled down by Rpd3-FLAG
IP-MS detection of proteins pulled down by Rpd3-FLAG by mass spectrometry
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-02-07 | PXD039051 | Pride
Rpd3 Saccharomyces cerevisiae，ip-ms 
TranscriptomicsTranscription profiling of S. cerevisiae RPD3 deletion mutants and histone H3 and H4 amino-terminus mutants
Signal intensity data for rpd3 delete, H3delta(1-28), H3(K4,9,14,18,23,27Q), H4delta(2-26), H4(K5,8,12,16Q), rpd3 delete H3delta(1-28), and rpd3 delete H4(K5,8,12,16Q) yeast grown in rich (YPD) media
ORGANISM(S): Saccharomyces cerevisiae 
2007-07-09 | E-GEOD-1639 | biostudies-arrayexpress
ProteomicsPKA plays a conserved role in regulating gene expression and metabolic adaptation
PKA regulates metabolism adaptation by phosphorylating Rpd3/HDAC1
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-03-12 | PXD058634 | Pride
Pka hdac1 rpd3 
ProteomicsThe Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation
In eukaryotes, trimethylation of lysine 9 on histone H3 (H3K9) is associated with transcriptional silencing of transposable elements (TEs). In drosophila ovaries, this heterochromatic repressive mark is thought to be deposited by SetDB1 on TE genomic loci after the initial recognition of nascent tra...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2020-07-21 | PXD018749 | Pride
Mep-1 Rpd3 Mi-2 Gtsf1 
TranscriptomicsRPD3-Q3451.Kc
modENCODE_submission_3296 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under s...
ORGANISM(S): Drosophila melanogaster 
2011-10-12 | E-GEOD-32765 | biostudies-arrayexpress
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