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ProteomicsRNA pull down experiment for vA-202 (ANAPC1P2) lncRNA
This study aimed to identify protein interaction partners of the lncRNA vA-202 using an RNA pull-down approach followed by mass spectrometry. Biotinylated vA-202 RNA was generated by in vitro transcription incorporating biotin-14-CTP. For each pull-down reaction, 400 µg of whole-cell lysate (in a to...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD070150 | Pride
Human Rna pull down Orbutrap ms 
GenomicsProteomic and Genomic Analysis of transcription factor TFII-I reveals insight into the response to cellular stress
We expressed biotin-tagged transcription factor TFII-I (GTF2I) in K562 cells and subjected crosslinked chromatin to streptavidin mediated pull down. We subjected the isolated DNA to Illumina sequencing. We identified 18920 TFII-I binding peaks. Nearest gene analysis revealed 5886 genes associated w...
ORGANISM(S): Homo sapiens 
2014-05-14 | E-GEOD-51065 | biostudies-arrayexpress
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2020-05-27 | PXD016576 | Pride
Streptavidin Affinity purification Mass spectrometry Dna pull down Chip sicap Lipid binding protein Bioid Membrane protein Chip Biotin 
ProteomicsHaCaT ACSL4mRNA probe pull-down MS
ACSL4mRNA probe was designed for pull-down experiments in HaCaT lysates
ORGANISM(S): Homo sapiens (Human) 
2025-10-13 | PXD052755 | Pride
Hacat acsl4mrna pull-down ms 
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-04-08 | PXD015858 | Pride
Lipid Mass spectrometry Interactors Streptavidin Membrane proteins Chip-sicap Biotinylation Bioid Chip Ceramide-binding proteins 
TranscriptomicsRNA-biotin based pull down assay with polyA site (PAS) RNA substrates in Hela Nuclear Extract followed by RNA-seq to discover trans-acting RNA molecules involved in mRNA 3 processing
We aimed to discover trans-acting RNA molecules involved in mRNA 3 processing. We reasoned that, if there exist such functional RNAs, they must directly associate with the key machinery responsible for mRNA 3 processing. Therefore, it would be of great value to comprehensively identify RNAs interact...
ORGANISM(S): Homo sapiens 
2017-06-01 | E-MTAB-5383 | biostudies-arrayexpress
GenomicsRNA pull-down analysis of hsa_circ_0001400 in 293T cells
RNA pull-down analysis of hsa_circ_0001400 in 293T cells
PRJNA852785 | ENA
GenomicsStreptavidin mediated CLEAR-CLIP in desmoplakin-BirA overexpressing mouse keratinocyte
Streptavidin mediated CLEAR-CLIP in desmoplakin-BirA overexpressing mouse keratinocyte
PRJNA1191528 | ENA
ProteomicscircMAN1A2_009-pulled down proteins in HeLa cells
CircRNA pulldown assays were conducted to identify proteins interacting with circMAN1A2_009. In summary, HeLa cell protein extracts were incubated with biotin-labeled circMAN1A2_009 probes obtained from Tsingke Biotechnology, China, at room temperature for 1h. Subsequently, streptavidin magnetic bea...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD052372 | Pride
Circman1a2_009 Hela Pull-down assays 
ProteomicsPull-down of nuclear proteins from HGC-27 cell line
To identify nuclear proteins that interact with KIT2KIT* G-quadruplex DNA, pull-down assays were performed with nuclear extracts from the KIT-positive HGC-27 cell line. Streptavidin-coated paramagnetic beads were derivatized with the biotinylated oligonucleotide and subsequently incubated with nucle...
ORGANISM(S): Homo sapiens (Human) Streptomyces avidinii 
2022-04-04 | PXD026505 | Pride
Lc-mse Nuclear extracts In-gel tryptic digests Hgc-27 cells Pull-down assays Protein identification 
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