Sort   by:  
 Page size 
TranscriptomicsA native chromatin purification system for epigenomic profiling in C. elegans
We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...
ORGANISM(S): Caenorhabditis elegans 
2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2020-05-27 | PXD016576 | Pride
Streptavidin Affinity purification Mass spectrometry Dna pull down Chip sicap Lipid binding protein Bioid Membrane protein Chip Biotin 
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-04-08 | PXD015858 | Pride
Lipid Mass spectrometry Interactors Streptavidin Membrane proteins Chip-sicap Biotinylation Bioid Chip Ceramide-binding proteins 
UnknownIsolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1.
Not available
S-EPMC4613318 | biostudies-literature
UnknownOptimized Lambda Exonuclease Digestion or Purification Using Streptavidin-Coated Beads: Which One Is Best for Successful DNA Aptamer Selection?
Not available
S-EPMC9680285 | biostudies-literature
ProteomicsChemical acetylation of ligands and two-step digestion protocol for reducing co-digestion in affinity purification-mass spectrometry
We present an effective, fast and user-friendly method to reduce co-digestion of bead-bound ligands, such as antibodies or streptavidin, in affinity purification-mass spectrometry experiments. A short pre-incubation of beads with Sulfo-NHS-Acetate leads to chemical acetylation of lysine residues, ma...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2023-10-23 | PXD043709 | Pride
Chemical acetylation Immunopurification Streptavidin Ligand Affinity purification Sulfo-nhs-acetate Nanobody Gfp 
UnknownEngineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling.
Not available
S-EPMC4583386 | biostudies-literature
Proteomicsiotin-streptavidin affinity purification mass spectrometry of proteins in human 293T cell using bioinylated Orf9b
COVID-19, caused by the new RNA virus strain SARS-CoV-2, is a serious acute respiratory disease. One of the prominent feature of SARS-COV-2 in terms of immune responses is that it triggers very low level responses of IFN-I in cells or COVID-19 patients. Previously SARS-CoV Orf9b were reported to be ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD019803 | Pride
Ap-ms Human 293t cell Silac Sars-cov-2 orf9b 
ProteomicsA novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics
Tandem-affinity purification mass spectrometry using the streptavidin-hemagglutinin (SH)-tag has been successfully employed to map signaling networks in several large-scale studies. However its application has so far been restricted to the small number of Flp/FRT-recombination competent cell lines....
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2016-01-05 | PXD002855 | Pride
Tandem affinity purification-mass spectrometry Protein-protein interaction Streptavidin-hemagglutinin-tag 
UnknownA monovalent streptavidin with a single femtomolar biotin binding site.
Not available
S-EPMC2576293 | biostudies-literature
Sort   by:  
 Page size