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ProteomicsThe quantitative proteomic analysis of Pseudomonas aeruginosa treated with or without Staphylococcus aureus-derived extracellular vesicles (SaEVs)
Pseudomonas aeruginosa was prepared and adjusted to an initial bacterial number of OD600 = 1.0 in Tryptic soy broth with or without 5 μg/mL SaEVs. After incubation at 37°C for 24 h, the bacterial cells were collected by centrifugation at 1,100 x g, 4°C for 15 min, followed by washing twice with phos...
ORGANISM(S): Pseudomonas Aeruginosa 
2024-01-30 | PXD043857 | JPOST Repository
ProteomicsProteomic analysis of extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)
PaOMVs in the culture supernatant of P. aeruginosa were harvested by ultracentrifugation at 100,000 × g, 4℃ for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removal of the supernatant, the pellet was washed twice and suspended in ice-cold sterile phosphate-b...
ORGANISM(S): Pseudomonas Aeruginosa 
2023-07-18 | PXD036377 | JPOST Repository
UnknownWestern-Type Helicobacter pylori CagA are the Most Frequent Type in Mongolian Patients.
Not available
S-EPMC6562502 | biostudies-literature
ProteomicsThe proteins identified from Staphylococcus aureus-derived extracellular vesicles (SaEVs)
Staphylococcus aureus strain ATCC 1718 was inoculated at 0.5% in 400 mL of Brain Heart Infusion broth for 8 h under aerobic conditions. The supernatant was collected by centrifugation twice at 5,000 x g, 4°C for 20 min, filtrated through a 0.45 µm filter to remove all remaining bacterial cells. S. a...
ORGANISM(S): Staphylococcus Aureus 
2024-01-30 | PXD043854 | JPOST Repository
ProteomicsProteomic analysis of Staphylococcus aureus after treating with extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)
For proteomic analysis of PaOMV-treated S. aureus, S. aureus (5 × 103 CFU/mL) was incubated with and without 1 µg/mL PaOMVs for 6 h at 37℃ under static condition and proteomic analysis was performed as described above. The identified proteins were searched against S. aureus ATCC1718 (NCBI Genebank: ...
ORGANISM(S): Staphylococcus Aureus 
2023-05-08 | PXD036381 | JPOST Repository
ProteomicsSalmon nasal cartilage proteoglycan up-regulates Listeria monocytogenese-mediated immune response in mice
The proteomic analysis of liver samples from mice administered proteoglycan (PG) for 9 days or untreated control mice. Liver samples from mice administered PG for 9 days or untreated control mice (n = 6 per group) were homogenized in PBS. The proteins were precipitated using acetone. The supernatant...
ORGANISM(S): Mus Musculus (mouse) 
2026-03-05 | PXD061495 | JPOST Repository
ProteomicsProteomic analysis of bone marrow-derived macrophages of mice treated with recombinant Ssa1, a protein enriched in the hypoxic secretome of Candida albicans.
Bone marrow-derived macrophages from mice were treated with recombinant Ssa1, a protein enriched in the hypoxic secretome of Candida albicans.
ORGANISM(S): Mus Musculus (mouse) 
2024-01-29 | PXD046766 | JPOST Repository
ProteomicsProteome analysis of secretory substances containing proteins, extracellular vehicles, and others secreted from Candida albicans cultured under normal and hypoxic conditions.
Candida albicans were cultured under normal and hypoxic conditions and collected secretome from the culture supernatant with centrifugation. The secretome contains secretory proteins, extracellular vehicles, and others.
ORGANISM(S): Candida Albicans 
2024-01-29 | PXD046763 | JPOST Repository
ProteomicsThe proteins identified from Helicobacter pylori strain TN2wt-derived extracellular vesicle (HpEV-TN2wt)
Helicobacter pylori strain TN2wt was suspended in Brucella broth supplemented with 5% depleted exosomes FCS. The initial optical density at 600 nm of the bacterial suspension was adjusted to 0.02 in 200 mL of Brucella broth + 0.5% depleted exosome FCS. H. pylori was grown in a 500 mL flask under mic...
ORGANISM(S): Helicobacter Pylori 
2025-03-17 | PXD056531 | JPOST Repository
ProteomicsThe proteins identified from Helicobacter pylori strain Tx30a-derived extracellular vesicles (HpEVs-Tx30a)
Helicobacter pylori strain ATCC51932 (Tx30a) was suspended in Brucella broth supplemented with 5% depleted exosomes FCS. The initial optical density at 600 nm of the bacterial suspension was adjusted to 0.02 in 200 mL of Brucella broth + 0.5% depleted exosome FCS. H. pylori was grown in a 500 mL fla...
ORGANISM(S): Helicobacter Pylori 
2025-03-17 | PXD056530 | JPOST Repository
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