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ProteomicsTudor-based Proteomic Strategy Pan-specifically Enriches and Identifies Protein Arginine Methylation
In current study, we developed a molecular affinity strategy based on the Tudor domain of SMN, a naturally occurring methylarginine reader protein, for comprehensive proteomic profiling of cellular arginine methylation. We demonstrated that the Tudor domain-based approach exhibits broad specificity ...
ORGANISM(S): Homo sapiens (Human) 
2025-12-15 | PXD053390 | Pride
Proteomics Arginine methylation Molecular affinity-enrichment method Eif3d 
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry as an alternative to liquid chromatography-tandem mass spectrometry for top-down proteomics of histones with the identification of over 1000 histone proteoforms
Top-down proteomics (TDP) is an ideal approach for deciphering the histone code and it routinely employs reversed-phase liquid chromatography (RPLC)-tandem mass spectrometry (MS/MS). Here we present capillary zone electrophoresis (CZE)-MS/MS via the electro-kinetically pumped sheath-flow CE-MS inter...
ORGANISM(S): Bos taurus (Bovine) 
2022-04-04 | PXD021889 | Pride
Intact histone proteoforms/mobility prediction/top-down proteomics/ histone postlational modifications/calf histone 
ProteomicsDoxycycline-induced SUN2 overexpression in Y-201 MSC cell line
Cells resident in tissues must be resilient to the physical demands of their surroundings. Our current understanding of cellular mechano-signalling is largely based on static systems, but these models do not reproduce the dynamic nature of living tissue. Here, we examined the time-resolved response ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-24 | PXD012863 | Pride
Sun2 Msc Overexpression 
ProteomicsPrimary human MSCs treated with two different RNAi against SUN2
Cells resident in tissues must be resilient to the physical demands of their surroundings. Our current understanding of cellular mechano-signalling is largely based on static systems, but these models do not reproduce the dynamic nature of living tissue. Here, we examined the time-resolved response ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-24 | PXD012948 | Pride
Sun2 Msc Rnai 
ProteomicsPrimary Human MSCs subjected to 1 hr intense cyclic tensile strain with/without 24 hr recovery
Cells resident in tissues must be resilient to the physical demands of their surroundings. Our current understanding of cellular mechano-signalling is largely based on static systems, but these models do not reproduce the dynamic nature of living tissue. Here, we examined the time-resolved response ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-24 | PXD012949 | Pride
Strain Msc Flexcell 
ProteomicsProteome changes to primary human MSCs subjected to high-intensity cyclic tensile strain (CTS) aligned to SUN2 overexpressing samples
Our current understanding of cellular mechano-signalling is based on static models, which do not replicate the dynamics of living tissues. Here, we examined the time-dependent response of primary human mesenchymal stem cells (hMSCs) to cyclic tensile strain (CTS). At low-intensity strain (1 hour, 4%...
ORGANISM(S): Homo sapiens (Human) 
2019-04-24 | PXD012873 | Pride
Msc Cts 
ProteomicsNext generation unnatural monosaccharides reveal that ESRRBO GlcNAcylation enhances pluripotency of mouse embryonic stem cells
Unnatural monosaccharides such as azidosugars that can be metabolically incorporated into cellular glycans are currently used as a major tool for glycan imaging and glycoproteomic profiling. As a common practice to enhance membrane permeability and cellular uptake, the unnatural sugars are per O ac...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-09-09 | PXD015035 | Pride
1 1 3-pr2galnaz Esrrb O-glcnac 3-ac2galnaz 
ProteomicsNext generation unnatural monosaccharides reveal that ESRRBO GlcNAcylation enhances pluripotency of mouse embryonic stem cells
Unnatural monosaccharides such as azidosugars that can be metabolically incorporated into cellular glycans are currently used as a major tool for glycan imaging and glycoproteomic profiling. As a common practice to enhance membrane permeability and cellular uptake, the unnatural sugars are per O ac...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-09-12 | PXD014785 | Pride
1 1 3-pr2galnaz Esrrb O-glcnac 3-ac2galnaz 
ProteomicsMina53 demethylates histone 4 arginine 3 asymmetric di-methylation to regulate neural stem/progenitor cell identity
Arginine methylation of histones plays a critical role in regulating gene expression. The writers (methyltransferases) and readers of methylarginine marks are well-known, but the erasers－arginine demethylases－remain mysterious. Here we identify Myc-induced nuclear antigen 53 (Mina53), a jumonji C do...
ORGANISM(S): Homo sapiens (Human) 
2024-10-22 | PXD045247 | Pride
Histone methylation Neural stem/progenitor cells Epigenetics Arginine demethylation Mina53 
MetabolomicsMetabolomic insights into the browning of the peel of bagging 'Rui Xue' apple fruit

BACKGROUND: Bagging is one of the most important techniques for the production of high-quality fruits. In the act of cultivation, we found a new kind of browning in peel of the apple fruit that occurs before harvest and worsens during the storage period. There are many studies on...

2021-04-09 | MTBLS2384 | MetaboLights
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