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ProteomicsHuman BMSCs protein analysis that interact with TRAF4 during adipogenic differentiation
To explore the mechanism underlying the negative regulation of adipogenesis by TRAF4, we performed the LC-MS/MS experiments to identify the proteins that interact with TRAF4 during adipogenic differentiation.
ORGANISM(S): Homo sapiens (Human) 
2020-05-27 | PXD017524 | Pride
Human Mscs Traf4 Adipogenic 
ProteomicsBRD4 regulates the osteogenic differentiation of MSCs
MSCs were lysed in cell lysis buffer for Western blotting and IP (Beyotime, Cat. No. P0013). A Dynabeads™ protein G immunoprecipitation kit (Invitrogen, Cat. No. 10007D) was used for Co-IP according to the manufacturer’s instructions. Dynabeads were resuspended and placed on a magnet to remove the s...
ORGANISM(S): Homo Sapiens 
PXD034615 |
ProteomicsMethylcobalamin protects against liver failure via engaging gasdermin E
To probe the binding sites of MeCbl on GSDME, 0.4 μM recombinant human GSDME (CSB-EP006766HU) purchased from CUSABIO (Wuhan, China) was incubated with 10 μM MeCbl in 100 μL buffer (20 mM HEPES, 150 mM NaCl and 50 μM DPA) at room temperature for 2 h and protected from light. 0.4 μM recombinant human ...
ORGANISM(S): Homo Sapiens 
2024-11-11 | PXD057768 |
ProteomicsChemoproteomic Mapping of the Glycolytic Targetome in Cancer Cells
we report a Target Responsive Accessibility Profiling (TRAP) approach for the global and quantitative analysis of glycolytic metabolites targetome in native cancer cell milieu.
ORGANISM(S): Homo Sapiens 
2020-11-17 | PXD022568 |
ProteomicsInvestigating the off-target effects of Pro-BA using label-free and TMTpro quantitative proteomics
Nevertheless, in-depth characterization of the proteome could reveal unknown off-targets of Pro-BA. To address this issue, we repeated the same experiment but extended MS analysis time to 2 hours in this revision, which will be elaborated in the next point. Nonetheless, the computed p-value of ALK p...
ORGANISM(S): Homo Sapiens 
2025-03-25 | PXD062245 |
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