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In aquatic environments, the production and consumption of organic compounds is directly tied to the metabolic potential of the in situ microbial community. The community’s metabolic potential can be assessed using metatranscriptomics, which is a measure of gene expression in the environment. More r...
2016-01-14 | MTBLS293 | MetaboLights

BACKGROUND AND AIMS: Atherosclerosis, characterized by dysfunction of lipid metabolism and chronic inflammation, is considered as a metabolic disorder as well as a major contributor to cardiovascular diseases. However, metabolic signatures of atherosclerosis have not been well ex...

2024-09-26 | MTBLS9737 | MetaboLights
Surface shaving Mtb using two concentrations of trypsin: 0.4 ug/mL and 0.05 ug/mL. Both bacteria and supernatant without the bacteria were subject to trypsinization, to allow for a ratio between samples to identify candidate surface proteins.
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2024-12-11 | PXD052936 | Pride
In-situ glial cell-surface proteomics identifies pro-longevity factors in Drosophila
We have developed a multi-analyte fluid-phase protein array technology termed high-throughput immunophenotyping using transcription (HIT). This method employs a panel of monoclonal antibodies, each tagged with a unique oligonucleotide sequence that serves as a molecular barcode. After staining a s...
ORGANISM(S): Homo sapiens 
Cell Surface Capture (Wollscheid et al., 2009, Nat.biotec.) was performed on various different cell types in order to characterize the cell surface proteome and to build the Cell Surface Protein Atlas.
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2016-02-16 | PXD000589 | Pride
The extracellular vesicle (EV) surface proteome (surfaceome) acts as a fundamental signalling gateway by bridging intra-and extracellular signalling networks, dictates EVs’ capacity to communicate and interact with its the/their environment, and is a source of potential disease biomarkers and thera...
ORGANISM(S): Homo sapiens (Human) 
2021-11-17 | PXD026658 | Pride
Large numbers of cells are generally required for quantitative global proteome profiling due to the significant surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic profiling impossible...
ORGANISM(S): Homo Sapiens 
2021-03-11 | PXD022827 | panorama
Monocytes are a critical component of the cellular innate immune system, and can be subdivided into classical, intermediate and non-classical subsets on the basis of surface CD14 and CD16 expression. Classical monocytes play the canonical role of phagocytosis, and account for the majority of circula...
ORGANISM(S): Homo sapiens (Human) 
2020-03-03 | PXD013832 | Pride
Cornea transplantation is a widely performed procedure globally. However, storing donor corneas for extended periods in Optisol-GS storage medium at 4 ℃ can lead to reduced endothelial cell viability, causing severe stromal edema and opacification. This negatively impacts transplantation success. Th...
ORGANISM(S): Homo Sapiens (human) 
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