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ProteomicsHuman cancer cell MCF-7 IP-PTEN MS
The MCF-7 cells were lysed using RIPA lysis buffer with a high salinity content to break out non-covalent binding of proteins. Then IP under partially denaturing conditions was performed and demonstrated that anti-PTEN precipitates were detected as smear bands, then mass spectrometry analysis of the...
ORGANISM(S): Homo sapiens (Human) 
2022-04-04 | PXD021544 | Pride
Human Breast cancer Pten Ms 
ProteomicsMCF-7 PTEN-Nedd8 IP-MS
We intended to elucidate the underlying mechanism of PTEN-Nedd8 in tumorigenesis. So, we firstly employed affinity purification and mass spectrometry to interrogate PTEN-Nedd8 interactome in nucleus.
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD011395 | Pride
Human Breast cancer cell Nedd8 Pten Ip-ms 
ProteomicsMCF-7 Flag-need8 IP-MS
To allow the proteomic identification of proteins modified with Nedd8, we firstly employed affinity purification and mass spectrometry to interrogate Nedd8 interactome.
ORGANISM(S): Homo sapiens (Human) 
2022-04-01 | PXD011368 | Pride
Human Breast cancer cell Ip-ms Mcf-7 
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