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ProteomicsCharacterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation
Heat shock protein 90 (HSP90) is a highly abundant molecular chaperone that interacts with many other intracellular proteins to regulate various cellular processes. However, compositions of the HSP90-interacting complex remain underinvestigated. This study thus aimed to characterize such complex in ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-12-17 | MSV000083252 | MassIVE
ProteomicsIdentification of Mitogen-Activated Protein Kinase Phosphatase-1 (MKP-1) protein partners using Tandem Affinity Purification and Mass Spectrometry
According to the World Health Organization Report, depressive disorders affect about 10% of the population. The molecular mechanism of the pathogenesis of depression is still not well understood. The new findings point to phosphatases as potential targets for effective depression therapy. The aim of...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) 
2023-01-19 | MSV000091093 | MassIVE
TranscriptomicsA toolbox for epitope-tagging and genome-wide location analysis in Candida albicans
Background : Candida albicans is a diploid pathogenic fungus not yet amenable to routine genetic investigations. Understanding aspects of the regulation of its biological functions and the assembly of its protein complexes would lead to further insight into the biology of this common disease-causing...
ORGANISM(S): Candida albicans 
2010-05-16 | E-GEOD-13427 | biostudies-arrayexpress
ProteomicsA novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics
Tandem-affinity purification mass spectrometry using the streptavidin-hemagglutinin (SH)-tag has been successfully employed to map signaling networks in several large-scale studies. However its application has so far been restricted to the small number of Flp/FRT-recombination competent cell lines....
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2016-01-05 | PXD002855 | Pride
Tandem affinity purification-mass spectrometry Protein-protein interaction Streptavidin-hemagglutinin-tag 
GenomicsBenchmark study of different chromatin isolation methods using the E2Fa transcription factor
Genome-wide identification of transcription factor (TF) binding sites is pivotal to our understanding of gene expression regulation. Although much progress has been made in the determination of potential binding regions of proteins by chromatin immunoprecipitation (ChIP), this method has some inhere...
ORGANISM(S): Arabidopsis thaliana 
2014-02-04 | E-GEOD-53422 | biostudies-arrayexpress
TranscriptomicsRIP-Seq analysis of Trypanosoma brucei ELAV-like protein Tb927.8.6650
A tagged ectopic version of the ELAV-like protein Tb927.8.6650 of T. brucei was expressed in stable cell lines and pulled down. Co-purifying transcripts were analyzed by sequencing to identify RNAs associated with Tb927.8.6650. Stable procyclic form cell lines expressing tetracycline-inducible TAP-t...
ORGANISM(S): Trypanosoma brucei 
2013-04-18 | E-GEOD-46160 | biostudies-arrayexpress
ProteomicsIntegrated Tandem Affinity Protein Purification Using the Polyhistidine plus Extra 4 Amino Acids (HiP4) Tag System
Interactome analysis of HBx using a novel tag system (HiP4-tag system).
ORGANISM(S): Homo Sapiens (human) 
2023-02-28 | PXD036256 | JPOST Repository
ProteomicsIntegrated Tandem Affinity Protein Purification Using the Polyhistidine plus Extra 4 Amino Acids (HiP4) Tag System２
Interactome analysis of TARDBP (TDP-43) using a novel tag system (HiP4-tag system).
ORGANISM(S): Homo Sapiens (human) 
2023-02-28 | PXD038445 | JPOST Repository
ProteomicsTandem affinity purification for sigma3 interacting host proteins
In order study the mechanism of how sigma3 regulates NF-kB activation, we performed tandem affinity purification to identify its host interacting proteins.
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD061062 | Pride
Tandem affinity purification Reovirus Ms 
GenomicsGenome-Wide Identification of the FRS12 Binding Sites by Tandem Chromatin Affinity Purification - Seq Analysis in Arabidopsis thaliana
Genome-Wide Identification of the FRS12 Binding Sites by Tandem Chromatin Affinity Purification - Seq Analysis in Arabidopsis thaliana
PRJEB7532 | ENA
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