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We investigated the N-terminal domain of PRDM14, which has no discernable functional domain, and its contribution to the maintenance of pluripotency. We performed a mass spectrometry analysis of the PRDM14-containing complex to disclose partner molecules that bind to the N-terminal domain of PRDM14.
ORGANISM(S): Mus Musculus (mouse) 
Mass spectrometry analysis of NeuN-positive neuronal nuclei from cortices of young and aged mice
ORGANISM(S): Mus Musculus (mouse) 
MEAF6 is essential for proliferation and plays a role in the assembly of KAT7 complexes.
ORGANISM(S): Mus Musculus (mouse) 
Mass spec analysis was performed to identify interacting proteins with FLAG-tagged Mettl1 in Drosophila ovary.
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
Synthetic phosphopeptides and enriched K562 cell phosphopeptides were analyzed using a TripleTOF 6600 mass spectrometer before and after enzymatic dephosphorylation. Dephosphorylated peptides identified by X!Tandem database searching were subjected to spectral simulation of all possible single phosp...
ORGANISM(S): Homo Sapiens (human) 
Mass spectrometry analysis of HSF5-interacting proteins from mouse testis chromatin
ORGANISM(S): Mus Musculus (mouse) 
Anti-TEX30 antibody-based comparative interactome analysis of TEX30
ORGANISM(S): Mus Musculus (mouse) 
(1) Secreted materials from the venom gland: 50 venom glands were cultured in 20 μL PBS for 2 hours. The cultured media were collected. Three biological replications were analyzed. (2) Lysates of the venom glands: 50 venom glands were squashed in 50 μL PBS and centrifuged. The supernatants were coll...
ORGANISM(S): Cellular Organisms 
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