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ProteomicsPharmacoproteomic profiling directed by C. elegans-driven phenotypic quantitative HTS
Quantitative high-throughput screening (qHTS) pharmacologically evaluates libraries of drugs and investigational agents for potential therapeutic uses, toxicological risk assessment, and increasingly for academic chemical tool discovery. Phenotypic HTS assays aim to interrogate molecular pathways a...
ORGANISM(S): Caenorhabditis elegans 
2023-02-21 | PXD033552 | Pride
Model organisms Laser cytometry Proteomics Drug discovery Infectious disease Genetic disorders High-throughput screening 
ProteomicsPhenotypic quantitative high throughput screening with Caenorhabditis elegans
Quantitative high throughput screening (qHTS) pharmacologically evaluates libraries of drugs and investigational agents for potential therapeutic uses, toxicological risk assessment, and increasingly for academic chemical tool discovery. Phenotypic HTS assays aim to interrogate molecular pathways a...
ORGANISM(S): Caenorhabditis elegans 
2023-05-30 | PXD027733 | Pride
Model organisms Laser cytometry Drug discovery Infectious disease Genetic disorders High-throughput screening 
ProteomicsA Universal 384-Well Proteomic Sample Preparation Platform for High-Throughput Screening and Biomarker Discovery
Major advances have been made to improve the sensitivity of mass analyzers, spectral quality, and the speed of data processing enabling more comprehensive proteome discovery and quantitation. While focus has recently begun shifting toward robust proteomic sample preparation efforts, a high throughpu...
ORGANISM(S): Homo sapiens (Human) 
2021-06-14 | PXD023374 | Pride
High-throughput Hplc-ms/ms Proteomics Biomarker Compound screen 
ProteomicsData-Independent Acquisition Enhancement of a Competitive Activity-Based Protein Profiling Platform for Kinase Inhibitor Screening
Kinase inhibitors represent a vital class of therapeutic agents widely used in cancer research, immunology, and other disease areas. Mass spectrometry (MS) employing specially designed small molecule kinase-binding probes has become an essential strategy for identifying novel kinase drug targets. Wh...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD072729 | Pride
Proteomics Mass spectrometry Activity-based protein profiling Kinase Data-independent acquisition 
ProteomicsKinome-profiling enabled discovery of a LATS1/2 inhibitor with wound healing potential
System-level mapping of kinase inhibitors polypharmacology can uncover opportunities for drug repurposing and identify starting points for medicinal chemistry campaigns aimed at novel targets. Here, we describe a large-scale profiling of 257 kinase inhibitors that revealed domains of promiscuity, po...
ORGANISM(S): Homo sapiens (Human) 
2026-07-15 | PXD073488 | Pride
Human Proteomics Kinase selectivity profiling Kinase inhibitor 
ProteomicsA Universal 384-Well Proteomic Sample Preparation Platform for High-Throughput Screening and Biomarker Discovery
Major advances have been made to develop an automated universal 384-well plate sample preparation platform with high reproducibility and adaptability for extraction of proteins from cells within a culture plate. An in-solution digest strategy is employed to generate peptides from the extracted prote...
ORGANISM(S): Homo sapiens (Human) 
2021-06-14 | PXD019733 | Pride
Proteomics Hplc-ms Mrm High-throughput screening Biomarkers 
ProteomicsCo-dependent macrocyclic peptide interactions facilitate ipglycermide high affinity binding to phosphoglycerate mutases
The phylogenetic divergence between parasitic and mammalian phosphoglycerate mutases (PGMs) offers an opportunity for selective pharmacologic targeting of glycolysis in disease-causing organisms. With this aim we previously described ipglycermides, discovered by affinity selection from a vast nuclei...
ORGANISM(S): Brugia malayi Dirofilaria immitis Escherichia coli Onchocerca volvulus Caenorhabditis elegans 
2021-04-06 | PXD024074 | Pride
Phosphoglycerate mutase Proteomics Cyclic peptide Structure Interaction 
ProteomicsBioprinted 3D vascularized human placenta models recapitulate early and late stages of pregnancy
The placenta is a highly dynamic organ that supports the growth and development of the fetus throughout the different stages of pregnancy. Malfunction of the placenta causes disorders of the pregnancy, including preeclampsia and pre-term birth. The study of the pathophysiology of the placenta at dif...
ORGANISM(S): Homo sapiens (Human) 
2026-09-07 | PXD072837 | Pride
Lc-msms Human Placenta 3d model 
ProteomicsMethotrexate-based PROTACs as DHFR chemical probes
Methotrexate (MTX) is a potent inhibitor of dihydrofolate reductase (DHFR), where is it used as both an antineoplastic and an immunosuppressant. Mechanisms of MTX resistance in cancers include MTX polyglutamylation and upregulation of DHFR. A series of MTX-based PROteolysis TArgeting Chimeras (PROTA...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD030140 | Pride
Dhfr Targeted protein degradation Methotrexate Chemical probe Protac One carbon metabolism 
ProteomicsAuranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterificlation to E2 ubiquitin-conjugating enzymes
UBA1 is the primary E1 ubiquitin-activating enzyme, regulating stability and function of numerous proteins via initiating their ubiquitination. Decreased or insufficient ubiquitination can cause or drive aging and many deadly diseases. Therefore, a small-molecule UBA1 activity enhancer could have br...
ORGANISM(S): Homo sapiens (Human) 
2023-07-10 | PXD042558 | Pride
Ubiquitination Uba1 Auranofin 
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