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ProteomicsAnalysis of human sperm surface glycoproteins which can interact with galectin-1
Herein, we constructed a human lectin microarray with 60 human lectin and lectin-like proteins. All of the lectins and lectin-like proteins were purified from yeast, and most showed binding to human glycans. To demonstrate the applicability of the human lectin microarray, human sperm were probed on ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD003785 | Pride
Nlm Galectin-1 Human sperm 
ProteomicsHuman protein phosphorylation analysis
Mycobacterium tuberculosis (Mtb) protein kinase G (PknG) is a critical protein which can inhibit the maturation of phagosome and its fusion with the lysosome, providing Mtb’s latency and survival in the host. However, its signaling pathways and functions in the human body are still unclear and neede...
ORGANISM(S): Homo sapiens (Human) 
2018-10-10 | PXD009237 | Pride
Phosphorylation Human 
Proteomicsiotin-streptavidin affinity purification mass spectrometry of proteins in human 293T cell using bioinylated Orf9b
COVID-19, caused by the new RNA virus strain SARS-CoV-2, is a serious acute respiratory disease. One of the prominent feature of SARS-COV-2 in terms of immune responses is that it triggers very low level responses of IFN-I in cells or COVID-19 patients. Previously SARS-CoV Orf9b were reported to be ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD019803 | Pride
Ap-ms Human 293t cell Silac Sars-cov-2 orf9b 
ProteomicsThe Ser/Thr Protein Kinase Protein-Protein Interaction Map of M. tuberculosis
Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis, the leading cause of death among all infectious diseases. There are 11 eukaryotic-like serine/threonine protein kinases (STPKs) in Mtb, which are thought to play pivotal roles in cell growth, signal transduction and pathogenesi...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2017-06-06 | PXD006389 | Pride
Protein-protein interaction Cell wall Murc Ser/thr protein kinase 
ProteomicsPafA AEBSF-2 - Identification of Serine 119 as an Effective Inhibitor Binding Site of M
Identified AEBSF binding site of PafA by LC-MS/MS analysis.
ORGANISM(S): Escherichia coli 
2018-03-21 | PXD007045 | Pride
Pafa aebsf 
ProteomicsPafA-ABESF - Identification of Serine 119 as an Effective Inhibitor Binding Site of M
Identified AEBSF binding site of PafA by LC-MS/MS analysis.
ORGANISM(S): Escherichia coli 
2018-03-21 | PXD006930 | Pride
Pafa abesf 
ProteomicsIP-MS target protein identification
To identify the target of the monoclonal antibody, Immuoprecipitation (IP) was performed to pull-down target protein from cell culture extraction, corn or zebrafish tissue lysate. The IP product was validated on silver-stained SDS-PAGE and Western blot. The target band was cut out and performed LC-M...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Homo sapiens (Human) Zea mays (Maize) 
2020-05-26 | PXD011629 | Pride
Human Maize Zebrafish Protein identification 
Proteomicsm6A binding proteins identified by SPIDER assay
To assess whether SPIDER could identify modified nucleic acids binding proteins within a complex environment, such as the cellular milieu, we sought to identify N6-methyladenosine (m6A) binding proteins within a total cell lysate. We performed SPIDER assay by incubating biotin-ssRNAs（with and withou...
ORGANISM(S): Homo Sapiens 
2022-11-02 | PXD026519 |
ProteomicsLenalidomide binding proteins identified by SPIDER assay
To assess whether SPIDER could capture protein-small molecule interaction in a complex environment, here we carried out the SPIDER assay by incubating biotin-Lenalidomide or a biotin control with HEK293T total lysate, followed by the use of biotin conjugated beads coupled with mass spectrometry to i...
ORGANISM(S): Homo Sapiens 
2021-06-04 | PXD026523 |
ProteomicsPupylation sites on protein Sox2
As a test for the effectiveness of SPIDER with RNA-protein pairs, We used Sox2 and specific biotin-DNA and biotin-RNA to validate the efficiency of SPIDER capturing protein-nucleic acid interaction. Pupylation sites on Sox2 after SPIDER assay was identified by LC-MS/MS analysis, by searching for an ...
ORGANISM(S): Homo Sapiens 
2021-06-04 | PXD026517 |
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