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ProteomicsAnalysis of human sperm surface glycoproteins which can interact with galectin-1
Herein, we constructed a human lectin microarray with 60 human lectin and lectin-like proteins. All of the lectins and lectin-like proteins were purified from yeast, and most showed binding to human glycans. To demonstrate the applicability of the human lectin microarray, human sperm were probed on ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD003785 | Pride
Nlm Galectin-1 Human sperm 
ProteomicsHuman protein phosphorylation analysis
Mycobacterium tuberculosis (Mtb) protein kinase G (PknG) is a critical protein which can inhibit the maturation of phagosome and its fusion with the lysosome, providing Mtb’s latency and survival in the host. However, its signaling pathways and functions in the human body are still unclear and neede...
ORGANISM(S): Homo sapiens (Human) 
2018-10-10 | PXD009237 | Pride
Phosphorylation Human 
Proteomicsiotin-streptavidin affinity purification mass spectrometry of proteins in human 293T cell using bioinylated Orf9b
COVID-19, caused by the new RNA virus strain SARS-CoV-2, is a serious acute respiratory disease. One of the prominent feature of SARS-COV-2 in terms of immune responses is that it triggers very low level responses of IFN-I in cells or COVID-19 patients. Previously SARS-CoV Orf9b were reported to be ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD019803 | Pride
Ap-ms Human 293t cell Silac Sars-cov-2 orf9b 
ProteomicsThe Ser/Thr Protein Kinase Protein-Protein Interaction Map of M. tuberculosis
Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis, the leading cause of death among all infectious diseases. There are 11 eukaryotic-like serine/threonine protein kinases (STPKs) in Mtb, which are thought to play pivotal roles in cell growth, signal transduction and pathogenesi...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2017-06-06 | PXD006389 | Pride
Protein-protein interaction Cell wall Murc Ser/thr protein kinase 
ProteomicsPafA-ABESF - Identification of Serine 119 as an Effective Inhibitor Binding Site of M
Identified AEBSF binding site of PafA by LC-MS/MS analysis.
ORGANISM(S): Escherichia coli 
2018-03-21 | PXD006930 | Pride
Pafa abesf 
ProteomicsPafA AEBSF-2 - Identification of Serine 119 as an Effective Inhibitor Binding Site of M
Identified AEBSF binding site of PafA by LC-MS/MS analysis.
ORGANISM(S): Escherichia coli 
2018-03-21 | PXD007045 | Pride
Pafa aebsf 
ProteomicsIP-MS target protein identification
To identify the target of the monoclonal antibody, Immuoprecipitation (IP) was performed to pull-down target protein from cell culture extraction, corn or zebrafish tissue lysate. The IP product was validated on silver-stained SDS-PAGE and Western blot. The target band was cut out and performed LC-M...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Homo sapiens (Human) Zea mays (Maize) 
2020-05-26 | PXD011629 | Pride
Human Maize Zebrafish Protein identification 
ProteomicsMass spectrometry to exam noval receptors of the The Omicron Variant of SARS-CoV-2 RBD captured by SPIDER
We used SPIDER to examine the cell-surface receptors for specific ligands while these receptors were in their native membranes within intact cells in culture. Here we coupled the SPIDER assay with biotinylated RBD of the Omicron Variant of SARS-CoV-2 and biotinylated RBD of SARS-CoV-2. Then we perfo...
ORGANISM(S): Homo Sapiens Chlorocebus Sabaeus 
2022-01-18 | PXD031035 |
ProteomicsPupylation sites on protein GFP-PupE
Characterization of GFP-Pup after Pupylation assays by mass spectrometry.
ORGANISM(S): Mycobacterium Tuberculosis H37rv_2009 
2021-06-04 | PXD026478 |
ProteomicsCheA pupylation sites identified by SPIDER assay
We used CheAs and biotin-CheZ to validate the efficiency of SPIDER capturing protein-protein interaction. Pupylation sites on WT CheAs after SPIDER assay was identified by LC-MS/MS analysis, by searching for an additional mass of ~243 Da, which represents the three C-terminal residues of Pup, i. e.,...
ORGANISM(S): Escherichia Coli 
2021-06-04 | PXD026509 |
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