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TranscriptomicsTranscription profiling of human umbilical vein endothelial cells (HUVEC) treated with thrombin and leukotriene D4 (LTD4) with ethanol controls to delinate Cysteinyl leukotrienes mode of action
Cysteinyl leukotrienes (cysLT), i.e. LTC4, LTD4, and LTE4, are lipid mediators derived from the 5-lipoxygenase pathway. The cysLT receptors cysLT1-R and cysLT2-R are expressed on different target cells and mediate inflammatory reactions in tissue- and LT-R-specific ways. Though endothelial cells (EC...
ORGANISM(S): Homo sapiens 
2007-10-27 | E-GEOD-3589 | biostudies-arrayexpress
no omics definedTranscription profiling of thrombin stimulated human microvascular endothelial cell line-1 (HMEC1) over time and vs untreated cells
Thrombin exerts pleiotropic effects on the endothelium. Nevertheless, little is known about its capacity to generate endothelial microparticles, a hallmark of endothelial activation. To gain insight into the mechanisms involved in microparticles release, the human microvascular endothelial cell line...
ORGANISM(S): Homo sapiens 
2006-01-20 | E-MEXP-477 | biostudies-arrayexpress
TranscriptomicsTranslation State Array Analysis of Thrombin Stimulated Human Endothelial Cells
Confluent human umbilical vein endothelial cells (HUVECs) were exposed to Thrombin (2 U/mL) for 2 hours. Ribosomal profiling via gradient centrifugation and fractionation was used to separate monosome, or under-translated, and polysome, or actively translated, mRNA species that were then used to pr...
ORGANISM(S): Homo sapiens 
2010-07-01 | E-GEOD-4919 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat thrombin-stimulated vascular smooth muscle cells
The strength and duration of extracellular signal-regulated kinases 1/2 (ERK1/2) phosphorylation can define the phenotypic outcome of cells. Stimualtion of the proteinase-activated receptor (Par) triggers a Gi-dependent long-lasting transactivation of the epidermal growth factor receptor and subsequ...
ORGANISM(S): Rattus norvegicus 
2009-05-02 | E-GEOD-15841 | biostudies-arrayexpress
TranscriptomicsACE-Scan of the thrombin DNA aptamer on 10^10 Gen1 DNA microarrays with sodium and potassium buffers in two colors.
The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on ...
ORGANISM(S): synthetic construct 
2017-12-20 | E-MTAB-6378 | biostudies-arrayexpress
no omics definedTranscription profiling of human endothelial cells exposed to thrombin for 2 or 6 hours
Endothelial cells were exposed to thrombin (5 IU/ml) for two times (2 and 6 hours) and compared to untreated cells for the same periods of time.
ORGANISM(S): Homo sapiens 
2007-04-01 | E-MEXP-952 | biostudies-arrayexpress
ProteomicsIdentification C3 cleavage sites by alpha-Thrombin
Identification C3 cleavage sites by alpha-Thrombin. Goal of the experiment is the validation of predicted cleavage sites by HTPS
ORGANISM(S): Homo sapiens (Human) 
2021-02-04 | PXD022971 | Pride
Htps Cleavages identification 
ProteomicsHs_platelets_Phospho_Thrombin
Human platelets Phospho proteome, treated with thrombin
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005291 | PeptideAtlas
ProteomicsThrombin activation of the factor XI dimer is a multi-staged process for each subunit
Factor XI (FXI) is a protein in the intrinsic coagulation pathway that can be activated by two enzymes. In hemostasis, FXI is activated by thrombin, while FXIIa-mediated activation of FXI is thought to be prothrombotic. The interactions of these enzymes with FXI are poorly understood due to their tr...
ORGANISM(S): Homo sapiens (Human) 
2024-02-05 | PXD039489 | Pride
Molecular dynamics Factor xi Crosslinking mass spectrometry Phox Thrombin 
ProteomicsTarget quantification of octapeptide for Thrombin and FX
Proteases are crucial physiologic regulators of protein structure and function. While proteomic methods contributed extensively to protease characterization efforts, technical challenges remain in terms of throughput, scalability and large datasets of protease cleavages remain scarce. Here, we descr...
ORGANISM(S): Homo sapiens (Human) 
2021-02-04 | PXD020320 | Pride
High-throughput protease screen Substrates Blood coagulation proteases Specificity Entropy Allostery 
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