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ProteomicsIon mobility fractionation coupled to PASEF for enhanced proteome coverage
Data provided report the use of trapped ion mobility spectrometry (TIMS) to fractionate ions in the gas phase based on their ion mobility (V⋅s/cm2) followed by parallel accumulation serial fragmentation (PASEF) in a quadrupole time-of-flight instrument. TIMS fractionation coupled to DDA-PASEF allowe...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD033129 | Pride
Timstof pro Pasef Dia Segmented Tims fractionation Fractionation Optimized Deep coverage Dda Ion mobility 
UnknownEnhancement of Proteome Coverage by Ion Mobility Fractionation Coupled to PASEF on a TIMS-QTOF Instrument.
Not available
S-EPMC10653119 | biostudies-literature
UnknownNanoparticle-Assisted Uranium Extraction and Precise Isotopic Fingerprinting in Groundwater by TIMS.
Not available
S-EPMC12809863 | biostudies-literature
ProteomicsA Simplified Method for Comprehensive Capture of the Staphylococcus aureus Proteome
Staphylococcus aureus is major human pathogen causing severe and invasive infections in both community and healthcare settings. Although well studied, a comprehensive exploration of its dynamic and adaptive proteome is still somewhat lacking. Herein we employ streamlined liquid- and gas-phase fracti...
ORGANISM(S): Staphylococcus aureus 
2025-03-31 | PXD053994 | Pride
Gas-phase fractionation Proteomics Dia-pasef Dda-pasef Staphylococcus aureus Ion mobility 
ProteomicsOnline parallel accumulation – serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer
In bottom-up proteomics, peptides are separated by liquid chromatography with elution peak widths in the range of seconds, while mass spectra are acquired in about 100 microseconds with time-of-fight (TOF) instruments. This allows adding ion mobility as a third dimension of separation. Among several...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-11-06 | PXD010012 | Pride
Tims Pasef Technological 
ProteomicsExtended coverage of human serum glycosphingolipidome by 4D-RP-LC TIMS-PASEF unravels association with Parkinson's disease
Glycosphingolipids (GSLs) are important targets in immune, infectious, lysosomal storage diseases, cancer, and neurodegenerative diseases. Circulatory GSL profiling in clinical samples is restricted by the lack of mid- and high-throughput analytical methods and deep coverage of long-chain sialylated...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-01-31 | MSV000097015 | MassIVE
ProteomicsDeep proteome coverage of microglia using a streamlined data independent acquisition-based proteomic workflow: method consideration for a phenotypically diverse cell type
As the immune cells of the brain, microglia play a key role in various homeostatic and disease-related processes. In order to carry out their numerous functions, microglia are able to adopt a wide range of phenotypic states. The proteomic landscape represents a more accurate molecular representation...
ORGANISM(S): Mus musculus (Mouse) 
2025-03-31 | PXD056436 | Pride
Dia-pasef Dda library generation Deep proteomics Microglia Dia methods development 
UnknownUltrafast and Reproducible Proteomics from Small Amounts of Heart Tissue Enabled by Azo and timsTOF Pro.
Not available
S-EPMC8349881 | biostudies-literature
ProteomicsProteomic Investigation of Plant Mitochondria lacking the Multiple Organellar RNA Editing Factor 3 (MORF3)
We compared the proteomes of mitochondria isolated from Arabidopsis thaliana wild-type and morf3-1 mutant line liquid cell culture (GK-109E12.01). Proteins were extracted and digested via the SP3 protocol. Peptides were cleaned up via solid-phase extraction. All samples were measured with three diff...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2025-02-21 | MSV000097179 | MassIVE
UnknownConcerns about Quadrupole ICP-MS Lead Isotopic Data and Interpretations in the Environment and Health Fields.
Not available
S-EPMC5923765 | biostudies-literature
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