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Proteomic analysis was conducted to identity proteins associated with the PilQ secretin complex in Pseudomonas aeruginosa. Samples were analyzed using a trapped ion mobility spectrometry time-of-flight (TIMS-TOF) mass spectrometer. The dataset includes raw mass spectrometry data and processed identi...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2026-06-01 | PXD077666 | Pride
We introduce a novel approach, termed time-segment acquisition (Seg), to enhance the identification of peptides and proteins in trapped ion mobility spectrometry (TIMS)-Time of flight (TOF) mass spectrometry. Our method exploits the positive correlation between ion mobility values and liquid chromat...
ORGANISM(S): Homo sapiens (Human) 
2024-12-22 | PXD051790 | Pride
Mitochondrial I+III2 supercomplex was purified from Arabidopsis thaliana cells using differential centrifugation including Percoll step gradients and continuous sucrose gradients. Supercomplex fractions were digested with trypsin using the SP3 approach. Peptides were further purified with SepPak car...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-12-30 | PXD036482 | Pride
Two-dimensional separation by nano-LC and trapped ion mobility spectrometry (TIMS) prior to Q-TOF tandem mass spectrometry significantly improves the accuracy of isobaric tag-based quantitation in proteome analysis without the need for additional measurement time for TIMS insertion. The obtained pea...
ORGANISM(S): Cellular Organisms 
Mass spectrometry lipidomics dataset analyzing degradation pathways of ionizable lipids in mRNA lipid nanoparticles (LNPs) exposed to environmental stressors. UHPLC–TIMS-TOF MS was used to characterize lipid oxidation products and structural degradation of DLin-MC3-DMA (MC3), SM-102, and ALC-0315 un...
ORGANISM(S): Cellular Organisms 
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