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MetabolomicsQuantitative metabolomics of plasma and tissue interstitial fluids from mice

This study presents quantitative metabolomic measurements of plasma and tissue interstitial fluids (IF) collected from healthy C57BL/6J mice. Using liquid chromatography–mass spectrometry (LC–MS) and external chemical standards, absolute concentrations were determined for over one hundred polar m...

2025-12-30 | MTBLS13165 | MetaboLights
MetabolomicsTime-dependent stability of plasma and liver interstitial fluid metabolites during sample handling in mice

This study assesses the stability of metabolite levels in plasma and liver interstitial fluid (IF) from mice subjected to different handling times prior to extraction. Blood and tissues from female C57BL/6J mice were collected and either processed immediately or held on ice for 5, 10, or 30 minut...

2025-12-30 | MTBLS13164 | MetaboLights
MetabolomicsUntargeted Metabolomics of the enthesis interstitial fluid of wild-type and Nlrp3-/- mice with sham operation and injury post 7 days
In order to investigate the effect of the NLRP3 inflammasome on the regeneration of enthesis, we established an enthesis injury and repair model in wild-type and NLRP3-/- mice. The interstitial fluid of enthesis was extracted, and untargeted metabolomics was conducted to discover differential metabo...
2025-07-08 | MTBLS12150 | MetaboLights
MetabolomicsMetabolic profiling of mouse tissues and tissue interstitial fluids
Not available
ORGANISM(S): Mus musculus 
ST002223 | MetabolomicsWorkbench
TranscriptomicsEarly Response Genes in Osteoblasts Exposed to Dynamic Fluid Flow
Cyclic mechanical loads applied to the skeleton increase bone formation. Dynamic fluid flow is a potent anabolic stimulus for cultured osteoblasts. In this study, gene profiles involved in mediating the anabolic response of MC3T3 osteoblasts to dynamic fluid flow are investigated. MC3T3 osteoblast R...
ORGANISM(S): Mus musculus 
2010-05-18 | E-GEOD-16211 | biostudies-arrayexpress
ProteomicsPeptide profiles in interstitial fluid of breast cancer samples with high or low CD10+ CAF infiltration
To investigate whether CD10 in CAFs exerts its functions by mediating peptide degradation, we applied a high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS)-based approach to compare peptide profiles in interstitial fluid of breast cancer samples with high or low CD10+ C...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD021590 | Pride
Breast cancer Lc-msms Human Tumor interstitial fluid 
ProteomicsEvaluation of tumor interstitial fluid-extraction methods for proteome analysis: comparison of biopsy elution versus centrifugation
The analysis of Tumor Interstitial Fluid (TIF) composition is a valuable procedure to identify anti-metastatic targets, and different laboratories have set up techniques for TIF isolation and proteomic analyses. However, those methods had never been compared in samples from the same tumor and patien...
ORGANISM(S): Homo sapiens (Human) 
2020-05-06 | PXD016261 | Pride
Cutaneous squamous cell carcinoma Skin cancer Tumor interstitial fluid Spin Proteome Centrifugation Elution 
ProteomicsProteomic profiling of paired interstitial fluids revealed dysregulated pathways and salivary NID1 as a biomarker of oral cavity squamous cell carcinoma
Patients with oral cavity squamous cell carcinoma (OSCC) are frequently first diagnosed at an advanced stage, leading to poor prognosis and high mortality rate. Early detection of OSCC using body fluid-accessible biomarkers may help improve the prognosis and survival rate of OSCC patients. As tumor ...
ORGANISM(S): Homo sapiens (Human) 
2019-08-15 | PXD008654 | Pride
Tumor interstitial fluid Saliva Oral cavity squamous cell carcinoma Aminoacyl trna biosynthesis Nidogen-1 Biomarker 
TranscriptomicsscRNA-seq analysis of cell subpopulations of the bronchoalveolar lavage fluid of healthy dogs
In dogs, a species for which markers of cell populations are often limiting, we sought to evaluate in an unbiased way the heterogeneity of cell subpopulations in the bronchoalveolar lavage fluid of healthy dogs, by single-cell RNA-sequencing.
ORGANISM(S): Canis lupus familiaris 
2020-06-29 | E-MTAB-9265 | biostudies-arrayexpress
ProteomicsBiomarkers found in the tumor interstitial fluid may help explain the differential behavior among keratinocyte carcinomas
Keratinocyte carcinomas (KC) are the most common malignancy worldwide. This category of tumours groups both basal (BCC) and squamous (SCC) cell carcinomas. While the former rarely metastasizes (0.03-0.55%), the latter is more associated to a substantial risk of local recurrence, metastasis and death...
ORGANISM(S): Homo sapiens (Human) 
2023-04-14 | PXD036900 | Pride
Orbitrap dusion lumos Basal cell carcinoma Squamous cell carcinoma Tumor interstitial fluid Label free 
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