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MetabolomicsPrediction and evaluation of the effect of pre-centrifugation sample management on the measurable untargeted LC-MS plasma metabolome

Optimal handling is the most important means to ensure adequate sample quality. We aimed to investigate whether pre-centrifugation delay time and temperature could be accurately predicted and to what extent variability induced by pre-centrifugation management can be adjusted for. We used untarget...

2021-10-13 | MTBLS2259 | MetaboLights
TranscriptomicsFluidigm Biomark 96.96 nanofluidic arrays for qRT-PCR of 93 miRNAs: salivary extracellular vesicles
Crude extracellular vesicles (EVs) from eight healthy volunteers were separated into 6 fractions based on their densities by using the iodixanol-based density gradient centrifugation method. To determine the distribution of miRNAs among these fractions, quantities of 93 miRNAs were quantified by the...
ORGANISM(S): Homo sapiens 
2016-08-01 | E-GEOD-78763 | biostudies-arrayexpress
ProteomicsEvaluation of tumor interstitial fluid-extraction methods for proteome analysis: comparison of biopsy elution versus centrifugation
The analysis of Tumor Interstitial Fluid (TIF) composition is a valuable procedure to identify anti-metastatic targets, and different laboratories have set up techniques for TIF isolation and proteomic analyses. However, those methods had never been compared in samples from the same tumor and patien...
ORGANISM(S): Homo sapiens (Human) 
2020-05-06 | PXD016261 | Pride
Cutaneous squamous cell carcinoma Skin cancer Tumor interstitial fluid Spin Proteome Centrifugation Elution 
ProteomicsThe Importance of Extracellular Vesicle Purification for Downstream Analysis: A Comparison of Differential Centrifugation and Size Exclusion Chromatography for Parasitic Helminths
Fascioliasis is a major neglected tropical disease that infects humans and ruminant species worldwide. Recent discoveries have identified that liver fluke extracellular vesicles are released into the host’s internal environment. To understand extracellular vesicle biology further, isolation of the e...
ORGANISM(S): Fasciola hepatica (Liver fluke) 
2019-02-13 | PXD008737 | Pride
Extracellular vesicles Size exclusion chromatography and fasciola hepatica. Purification Differential centrifugation 
ProteomicsComparative mass spectrometry analysis of high centrifugation vs. low centrifugation uEV pellets following THP removal
Urinary extracellular vesicles (uEVs) denote minuscule membranous entities generated by cells lining the urinary tract and adjoining tissues(1). The examination of uEVs as an exploratory instrument has garnered substantial focus owing to their prospective utility as non-invasive biomarkers for diagn...
ORGANISM(S): Homo sapiens (Human) 
2025-11-24 | PXD064844 | Pride
Urinary extracellular vesicles Fractionation Proteasome 
TranscriptomicsSubpopulation array data, Veening et al.
The transcriptome profiles of sporulating vs non-sporulating cells, within an isogenic culture were compared. RNA was isolated from endospore containing cells which were separated from non-spore forming cells using buoyant density gradient centrifugation within an isogenic culture (details in Veenin...
ORGANISM(S): Bacillus subtilis 
2010-05-25 | E-GEOD-9266 | biostudies-arrayexpress
UnknownCentrifugation-induced release of ATP from red blood cells.
Not available
S-EPMC6124747 | biostudies-literature
UnknownIdentification of the Centrifugation-Caused Paralytic Impact on Neutrophils.
Not available
S-EPMC12427856 | biostudies-literature
GenomicsLocolization of circRNA with DIS3 WT or D146N mutant in sucrose density gradient centrifugation assay
Locolization of circRNA with DIS3 WT or D146N mutant in sucrose density gradient centrifugation assay
PRJNA1079793 | ENA
UnknownEffects of Different Centrifugation Parameters on Equilibrium Solubility Measurements.
Not available
S-EPMC12566593 | biostudies-literature
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